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Rabbit抗原名称
Sp7/Osterix分子别名
Transcription factor Sp7; C22; Zinc finger protein osterix; Osx免疫原
Recombinant Protein细胞定位
NucleusAccession
Q8VI67克隆号
S-4225-14抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
mouse embryo, rat embryo纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:250 Ms, Rt IF 1:100-1:1000 Ms, Rt
Sp7/Osterix is a ~45 kDa zinc-finger transcription factor of the Sp/KLF family that is indispensable for osteoblast lineage commitment and bone formation, acting downstream of Runx2 and cooperatively with BMP-6 to drive expression of osteogenic genes such as COL1A1, ALP, OCN, SATB2 and SOST, while its own transcription is induced by BMP-2, IGF-I and PTH and its activity is tuned by Akt- and p38 MAPK-mediated phosphorylation, ubiquitin–proteasome degradation and autoregulation via an OSX-bound tandem repeat in its promoter, and genetic or regulatory disruption of this short-lived nuclear protein underlies skeletal disorders including osteogenesis imperfecta and osteoporosis.
免疫印迹
WB result of Sp7/Osterix Recombinant Rabbit mAb
Primary antibody: Sp7/Osterix Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse embryo lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 39, 48 kDaWB result of Sp7/Osterix Recombinant Rabbit mAb
Primary antibody: Sp7/Osterix Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat embryo lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 39, 48 kDa
免疫组化
IHC shows positive staining in paraffin-embedded normal mouse E14 embryo. Anti-Sp7/Osterix antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded normal mouse E14 embryo. Anti-Sp7/Osterix antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded normal mouse E14 embryo. Anti-Sp7/Osterix antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded normal rat E14 embryo. Anti-Sp7/Osterix antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded normal rat E14 embryo. Anti-Sp7/Osterix antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse E14 embryo. Anti-Sp7/Osterix antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse E14 embryo. Anti-Sp7/Osterix antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded Rat E14 embryo. Anti-Sp7/Osterix antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded Rat E14 embryo. Anti-Sp7/Osterix antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Sp7/Osterix in mouse & rat tissues.
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