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Mouse抗原名称
CD59分子别名
CD59 glycoprotein; 1F5 antigen; 20 kDa homologous restriction factor (HRF-20; HRF20); MAC-inhibitory protein (MAC-IP); MEM43 antigen; Membrane attack complex inhibition factor (MACIF); MIC11; MIN1; MIN2; MIN3; MSK21免疫原
Recombinant Protein细胞定位
Cell membrane, SecretedAccession
P13987克隆号
S-936-1抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
HeLa, HT-1080, U-87 MG, HT-29纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:2000 Hu
CD59, also known as protectin or membrane attack complex inhibitory factor (MACIF), is a glycosylphosphatidylinositol (GPI)-anchored membrane protein that plays a crucial role in regulating the complement system. It is widely expressed in most human tissues and acts as a major inhibitor of the membrane attack complex (MAC) formation. The mature CD59 protein consists of 77 amino acids and is anchored to the cell membrane via a GPI anchor. By binding to complement proteins C8 and C9, CD59 prevents the assembly of the MAC, thereby protecting host cells from complement-mediated lysis. Deficiencies in CD59 expression can lead to serious disorders such as paroxysmal nocturnal hemoglobinuria (PNH), characterized by the destruction of red blood cells. Additionally, CD59 is involved in T cell signal transduction and has been implicated in various diseases, including cancer and autoimmune disorders.
免疫印迹
WB result of CD59 Mouse mAb
Primary antibody: CD59 Mouse mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HT-1080 whole cell lysate 20 µg
Lane 4: U-87 MG whole cell lysate 20 µg
Lane 5: HT-29 whole cell lysate 20 µg
Negative control: Raji whole cell lysate
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 14 kDa
Observed MW: 17-24 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD59 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-CD59 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-CD59 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-CD59 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CD59 in tumor tissue.
Expression of CD59 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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