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宿主来源
Mouse抗原名称
HA tag克隆号
S-5404抗体同种型
IgG1,k纯化方式
Protein G浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:5000-1:100000 Species independent
The HA tag (Hemagglutinin tag) is an epitope tag derived from the hemagglutinin (HA) protein of human influenza virus, typically consisting of a 9-amino acid sequence (YPYDVPDYA). Widely used in molecular biology and biochemistry due to its high immunogenicity and specificity, this tag is primarily employed for the detection, purification, and localization of proteins. By genetically fusing the HA tag to the N- or C-terminus of a target protein, high-affinity anti-HA antibodies can be utilized in experiments such as Western blot, co-immunoprecipitation (Co-IP), and immunofluorescence (IF) to effectively track the expression, subcellular localization, and interactions of the target protein. With its small size, high sensitivity, and minimal interference with protein structure, the HA tag has become an essential tool in protein research.
免疫印迹
WB result of HA Tag Recombinant Mouse mAb
Primary antibody: HA Tag Recombinant Mouse mAb at 1/20000 dilution
Lane 1: 293T transfected with empty vector whole cell lysate 20 µg
Lane 2: 293T transfected with HA-tag fusion protein whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 30 kDa
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