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Rabbit抗原名称
N-Cadherin分子别名
Cadherin-2; CDw325; Neural cadherin (N-cadherin); CD325; CDHN; NCAD; CDH2免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P19022克隆号
SDT-329-12抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
1 mg/ml标记
Cy5性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
IF ?
稀释度
应用 稀释度 推荐种属 IF 1:500 Hu, Ms, Rt
CD325, also known as N-cadherin or cadherin-2, is a 130–140 kDa type I transmembrane glycoprotein belonging to the cadherin superfamily. It is a calcium-dependent cell-cell adhesion molecule that plays a crucial role in maintaining tissue architecture and regulating morphogenetic movements during development. The protein consists of five extracellular cadherin repeats, a transmembrane region, and a highly conserved cytoplasmic tail. The extracellular domains mediate homophilic interactions between adjacent cells, while the cytoplasmic tail interacts with catenin proteins, which link the cadherin complex to the actin cytoskeleton. CD325 is expressed in various tissues, including neurons, cardiac muscle, and some cancer cells, and is involved in processes such as neural development, cardiac function, and cancer metastasis.
免疫荧光
IF shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti-N-Cadherin Recombinant Rabbit mAb (Cy5 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded human chromophobe renal carcinoma. Anti-N-Cadherin Recombinant Rabbit mAb (Cy5 Conjugate) antibody was used at 1/500 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-N-Cadherin Recombinant Rabbit mAb (Cy5 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse liver. Anti-N-Cadherin Recombinant Rabbit mAb (Cy5 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat cardiac muscle. Anti-N-Cadherin Recombinant Rabbit mAb (Cy5 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat liver. Anti-N-Cadherin Recombinant Rabbit mAb (Cy5 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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