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宿主来源
Rabbit抗原名称
ROR beta/RORB分子别名
Nuclear receptor ROR-beta; Nuclear receptor RZR-beta; Nuclear receptor subfamily 1 group F member 2; Retinoid-related orphan receptor-beta; NR1F2; RZRB细胞定位
NucleusAccession
Q92753克隆号
S-5483抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Y79, mouse retina, rat retina纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu, Ms, Rt IHC-P 1:500 Ms, Rt
RORB, also known as RAR-related orphan receptor beta, is a member of the NR1 subfamily within the nuclear receptor superfamily. As a DNA-binding transcription factor, it recognizes and binds to ROR response elements (ROREs) in the promoter regions of downstream genes either as a monomer or homodimer, thereby regulating target gene transcription. Structurally, the protein contains a highly conserved DNA-binding domain (featuring two C4-type zinc fingers) and a C-terminal ligand-binding domain (containing the AF-2 activation function motif), with its transcriptional activity being inhibited by natural ligands such as all-trans retinoic acid (ATRA) through inverse agonism. The functions of RORB are highly tissue-specific and spatiotemporally regulated. In the retina, it is critical for the proper development and differentiation of both rod and cone photoreceptors, regulating photoreceptor cell fate through the induction of transcription factor pathways such as NRL. Additionally, it participates in modulating the periodicity and stability of circadian rhythms, and in the nervous system, it guides cortical neuron architecture and barrel cortex formation. Furthermore, different isoforms of RORB exhibit functional diversity—for example, isoform 1 is indispensable for hindlimb motor control and the differentiation of retinal amacrine and horizontal cells. Loss of function or mutations in the RORB gene have been associated with various diseases, including idiopathic generalized epilepsy (EIG15), myopia, and complex neurodevelopmental disorders.
免疫印迹
WB result of ROR beta/RORB Recombinant Rabbit mAb
Primary antibody: ROR beta/RORB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Y79 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDa
This blot was developed with high sensitivity substrate.WB result of ROR beta/RORB Recombinant Rabbit mAb
Primary antibody: ROR beta/RORB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse retina lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDaWB result of ROR beta/RORB Recombinant Rabbit mAb
Primary antibody: ROR beta/RORB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat retina lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDa
This blot was developed with high sensitivity substrate.
免疫组化
IHC shows positive staining in paraffin-embedded mouse retina. Anti-RORB antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse brain. Anti-RORB antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat retina. Anti-RORB antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat brain. Anti-RORB antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of RORB in tumor tissues.
Expression of RORB in human tissues.
Expression of RORB in mouse & rat tissues.
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