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宿主来源
Rabbit抗原名称
POU2F3分子别名
POU domain, class 2, transcription factor 3; Octamer-binding protein 11 (Oct-11); Octamer-binding transcription factor 11 (OTF-11); Transcription factor PLA-1; Transcription factor Skn-1; OTF11; PLA1细胞定位
NucleusAccession
Q9UKI9克隆号
S-5525抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
NCI-H526纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1: 1000 Hu IHC-P 1:100-1:200 Hu
POU2F3, also known as Oct-6 or Tst-1, is a transcription factor belonging to the class III POU domain family that plays a pivotal role in regulating gene expression during embryonic development and cellular differentiation, particularly within the nervous system and epidermal lineages. Characterized by its unique bipartite DNA-binding structure comprising a POU-specific domain and a POU homeodomain connected by a flexible linker, POU2F3 binds to specific octamer motifs in target gene promoters to modulate transcriptional activity, thereby driving critical processes such as Schwann cell myelination, neural crest formation, and the terminal differentiation of keratinocytes in the skin's suprabasal layers. Beyond its developmental functions, POU2F3 has garnered significant attention in oncology as a lineage-defining marker and potential therapeutic target in small cell lung cancer (SCLC) and other neuroendocrine tumors, where its aberrant overexpression contributes to tumor proliferation, survival, and the maintenance of a dedifferentiated state, making it a crucial molecule for understanding both normal tissue morphogenesis and malignant transformation mechanisms.
免疫印迹
WB result of POU2F3 Recombinant Rabbit mAb
Primary antibody: POU2F3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HCT 116 whole cell lysate 20 µg
Lane 2: NCI-H526 whole cell lysate 20 µg
Negative control: HCT 116 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 40-60 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-POU2F3 antibody was used at 1/125 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti-POU2F3 antibody was used at 1/125 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human ovarian clear cell carcinoma. Anti-POU2F3 antibody was used at 1/125 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of POU2F3 in tumor tissues.
Expression of POU2F3 in human tissues.
Expression of POU2F3 in mouse & rat tissues.
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