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宿主来源
Mouse抗原名称
Myc tag克隆号
S-5300抗体类型
Mouse mAb抗体同种型
IgG1纯化方式
Protein G浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:5000-1:50000 Species independent ICC 1:1000 Species independent
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
免疫印迹
WB result of Myc Tag Recombinant Mouse mAb
Primary antibody: Myc Tag Recombinant Mouse mAb at 1/50000 dilution
Lane 1: untreated 293T whole cell lysate 20 µg
Lane 2: 293T transfected with myc tag fusion protein whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 14 kDa
Observed MW: 14 kDa
免疫细胞化学
ICC shows positive staining in H3.3 (G34W mutant)-myc tag transfected 293T cells (top panel) and negative staining in vector-transfected 293T cells (below panel). Anti- myc tag antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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