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Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb (S-1529-102)

Glycogen synthase kinase-3 alpha,GSK-3 alpha,Serine/threonine-protein kinase GSK3A,GSK3A,Glycogen synthase kinase-3 beta,GSK-3 beta,Serine/threonine-protein kinase GSK3B,GSK3B

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6019

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Phospho-GSK3α/β (Ser21/9)
分子别名

Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B; GSK3B
免疫原

Synthetic Peptide
细胞定位

Cell membrane, Cytoplasm, Nucleus
Accession

P49840, P49841
克隆号

S-1529-102
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000 Hu, Ms, Rt

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000	Hu, Ms, Rt

背景介绍

Phospho-GSK3α/β (Ser21/9) refers to the phosphorylated forms of glycogen synthase kinase 3 alpha (GSK3α) at serine 21 and glycogen synthase kinase 3 beta (GSK3β) at serine 9. The phosphorylation at these sites is inhibitory, meaning it reduces the activity of these kinases. GSK3α/β are serine/threonine kinases that play crucial roles in various cellular processes, including cell fate determination, glucose homeostasis, and Wnt signaling. When phosphorylated at Ser21/9, the activity of GSK3α/β is suppressed, which can have significant impacts on these pathways. For example, in the Wnt signaling pathway, the inhibition of GSK3β by phosphorylation at Ser9 prevents the degradation of β-catenin, allowing it to accumulate and activate downstream gene expression. This phosphorylation is often regulated by upstream signals such as insulin or growth factors, which can induce the phosphorylation of GSK3α/β at these sites through various signaling cascades.

免疫印迹
- WB result of Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb
  Primary antibody: Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 47, 51 kDa
  Observed MW: 45, 51 kDa
- WB result of Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb
  Primary antibody: Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
  Lane 2: Serum-starved NIH/3T3 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 47, 51 kDa
  Observed MW: 45, 55 kDa
- WB result of Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb
  Primary antibody: Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated C6 whole cell lysate 20 µg
  Lane 2: C6 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 47, 51 kDa
  Observed MW: 47, 51 kDa
斑点杂交
- Dot blot result of Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb
  Lane 1: GSK-3β (Ser9) phospho peptide
  Lane 2: GSK-3β (Ser9) unmodified peptide
  Primary antibody: Phospho-GSK3α/β (Ser21/9) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution