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Rabbit抗原名称
VPS34分子别名
Phosphatidylinositol 3-kinase VPS34细胞定位
EndosomeAccession
Q6FSR7抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Raji, Jurkat, HepG2, PC-3, mouse brain, rat brain纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt IP 1:50 Hu, Ms, Rt IHC-P 1:50-1:250 Hu, Ms, Rt
VPS34, also known as PIK3C3, is the sole class III phosphatidylinositol 3-kinase in mammals and plays a central role in intracellular membrane trafficking, autophagy, and signal transduction. Its structure consists of an N-terminal C2 domain, a central PIK helical domain, and a C-terminal kinase catalytic domain, with the kinase domain specifically responsible for catalyzing the conversion of phosphatidylinositol (PtdIns) to phosphatidylinositol 3-phosphate (PI3P). It exerts its functions by forming distinct complexes with different regulatory subunits—such as VPS15 and Beclin 1—and their interaction partners: the complex I formed with ATG14 primarily regulates autophagosome formation, whereas the complex II formed with UVRAG mainly regulates endocytic trafficking and vesicular transport. The generated PI3P signaling molecule specifically recruits effector proteins containing FYVE or PX domains, thereby orchestrating key steps such as vesicle docking, membrane fusion, and endosomal sorting. Through these functions, VPS34 integrates nutrient sensing, cellular stress responses, and intracellular transport, making it essential for maintaining cellular homeostasis. Notably, dysregulation of VPS34 function has been linked to various pathological processes, including tumorigenesis and cardiovascular diseases.
免疫印迹
WB result of VPS34 Recombinant Rabbit mAb
Primary antibody: VPS34 Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 102 kDa
This blot was developed with high sensitivity substrateWB result of VPS34 Recombinant Rabbit mAb
Primary antibody: VPS34 Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 102 kDa
This blot was developed with high sensitivity substrateWB result of VPS34 Recombinant Rabbit mAb
Primary antibody: VPS34 Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 102 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-VPS34 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-VPS34 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-VPS34 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-VPS34 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-VPS34 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of VPS34 in tumor tissues.
Expression of VPS34 in human tissues.
Expression of VPS34 in mouse & rat tissues.
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