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宿主来源
Mouse抗原名称
OGT分子别名
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine transferase 110 kDa subunit (OGT)细胞定位
Cytoplasm, NucleusAccession
O15294抗体类型
Mouse mAb抗体同种型
IgG2a反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HEK-293, NIN/3T3, rat brain纯化方式
Protein A浓度
1.4 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:2000-1:10000 Hu, Ms, Rt IHC-P 1:250-1:1000 Hu, Ms, Rt
OGT is a widely expressed enzyme in the human body that primarily catalyzes O-GlcNAcylation, a dynamic and reversible post-translational modification. This modification involves the transfer of a single N-acetylglucosamine (GlcNAc) sugar moiety to serine or threonine residues of proteins, thereby participating in the regulation of various critical cellular processes, including signal transduction, gene expression, cell cycle progression, and stress responses. Structurally, the OGT protein contains an N-terminal domain consisting of 14 tetratricopeptide repeat (TPR) motifs and a C-terminal catalytic domain, where the TPR domain is responsible for recognizing and binding substrate proteins, while the catalytic domain transfers the sugar moiety from the UDP-GlcNAc donor to the target protein. Notably, UDP-GlcNAc is the end product of the hexosamine biosynthetic pathway (HBP), thus making OGT activity a direct reflection of the cellular nutritional status. In terms of pathology, OGT dysregulation is closely associated with various diseases: for instance, in cancer, OGT drives tumor initiation and progression by glycosylating multiple oncogene or tumor suppressor gene products; in neurodegenerative diseases, OGT modulates the function of neuro-related proteins such as tau through glycosylation, influencing disease progression; additionally, OGT also plays an important role in metabolic diseases, where alterations in its expression levels and activity are involved in regulating the expression of metabolism-related genes.
免疫印迹
WB result of OGT Mouse mAb
Primary antibody: OGT Mouse mAb at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 116 kDa
Observed MW: 116 kDaWB result of OGT Mouse mAb
Primary antibody: OGT Mouse mAb at 1/10000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 116 kDa
Observed MW: 116 kDaWB result of OGT Mouse mAb
Primary antibody: OGT Mouse mAb at 1/10000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 116 kDa
Observed MW: 116 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-OGT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-OGT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-OGT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-OGT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-OGT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of OGT in tumor tissues.
Expression of OGT in human tissues.
Expression of OGT in mouse & rat tissues.
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