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宿主来源
Mouse抗原名称
NDUFA9分子别名
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial; Complex I-39kD (CI-39kD); NADH-ubiquinone oxidoreductase 39 kDa subunit; NDUFS2L细胞定位
MitochondrionAccession
Q16795克隆号
S-5382抗体类型
Recombinant mAb抗体同种型
IgG1,k反应种属 ?
Hu, Ms, Rt阳性样本
WI-38, mouse heart, rat heart预测反应种属
(反应种属缩写表)Bv纯化方式
Protein G浓度
1.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:10000 Hu, Ms, Rt IHC-P 1:500 Hu, Ms, Rt
NDUFA9 is a mitochondrial protein encoded by the NDUFA9 gene, functioning as a 39-kDa non-catalytic accessory subunit of respiratory chain complex I. After being synthesized in the cytoplasm, this protein is transported to the mitochondrial matrix and is primarily localized to the Q module of complex I, where it plays a critical role in maintaining the structural stability and normal function of complex I. Although NDUFA9 is not directly involved in the catalytic electron transfer reactions, it is essential for the proper assembly of complex I and the stabilization of late assembly intermediates, and it may also participate in recruiting the substrate ubiquinone-10. At the pathological level, homozygous or compound heterozygous mutations in the NDUFA9 gene can lead to nuclear type 26 mitochondrial complex I deficiency (MC1DN26), a disorder inherited in an autosomal recessive manner with a broad clinical spectrum, ranging from neonatal-lethal Leigh syndrome to childhood-onset progressive neurodevelopmental disorders with prominent dystonia. Affected patients typically present with developmental delay, dystonia, seizures, and abnormal MRI signals in the basal ganglia, and the underlying pathogenesis lies in the failure of complex I assembly and a significant reduction in its activity caused by these mutations.
免疫印迹
WB result of NDUFA9 Recombinant mouse mAb
Primary antibody: NDUFA9 Recombinant mouse mAb at 1/1000 dilution
Lane 1: WI-38 whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 35 kDaWB result of NDUFA9 Recombinant mouse mAb
Primary antibody: NDUFA9 Recombinant mouse mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 35 kDaWB result of NDUFA9 Recombinant mouse mAb
Primary antibody: NDUFA9 Recombinant mouse mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 35 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-NDUFA9 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-NDUFA9 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-NDUFA9 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-NDUFA9 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of NDUFA9 in human tissues.
Expression of NDUFA9 in mouse & rat tissues.
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