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宿主来源
Rabbit抗原名称
Monoamine Oxidase B/MAOB分子别名
Amine oxidase [flavin-containing] B细胞定位
MitochondrionAccession
P27338克隆号
S-5317抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HepG2, MCF7, SH-SY5Y纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu IHC-P 1:250 Hu
Monoamine oxidase B (MAO-B) is an enzyme primarily found in the brain and platelets that catalyzes the oxidative deamination of neuroactive and vasoactive amines, particularly dopamine. By breaking down dopamine, MAO-B plays a key role in regulating its synaptic levels, making it an important pharmacological target—MAO-B inhibitors (e.g., selegiline, rasagiline) are used as adjunct therapies in Parkinson’s disease to enhance dopaminergic neurotransmission and provide neuroprotective effects.
免疫印迹
WB result of Monoamine Oxidase B/MAOB Recombinant Rabbit mAb
Primary antibody: Monoamine Oxidase B/MAOB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A204 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Negative control: A204 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 60 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-MAOB antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-MAOB antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-MAOB antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-MAOB antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-MAOB antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of MAOB in tumor tissues.
Expression of MAOB in human tissues.
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