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Mouse抗原名称
HNRNPA2B1分子别名
Heterogeneous nuclear ribonucleoproteins A2/B1; HNRPA2B1细胞定位
Secreted, Cytoplasm, NucleusAccession
P22626抗体类型
Mouse mAb抗体同种型
IgG2a反应种属 ?
Hu, Ms, Rt阳性样本
HEK-293, HeLa, K532, HepG2, NIH/3T3, C6纯化方式
Protein A浓度
1.8 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:5000-1:50000 Hu, Ms, Rt IHC-P 1:200-1:500 Hu, Ms, Rt ICC 1:500-1:1000 Hu, Ms, Rt
HNRNPA2B1 is a widely expressed RNA-binding protein in the human body, belonging to the hnRNP A/B subfamily. Its core function is to participate in the processing, transport, and metabolism of precursor mRNA, influencing gene expression regulation by binding to RNA to form ribonucleoprotein particles. This protein possesses a typical dual RNA recognition motif (RRM) domain, enabling it to specifically recognize and bind to specific sequences in RNA substrates, such as AGG and UAG motifs. HNRNPA2B1 is involved in mRNA splicing and nuclear export within the nucleus, and it has also been identified as a "reader" of N6-methyladenosine (m6A) modifications, recognizing primary miRNAs containing m6A marks and promoting their processing. Additionally, it participates in the cytoplasmic transport of specific mRNAs, the sorting of miRNAs into exosomes, and innate immune responses. Notably, aberrant expression or mutations of HNRNPA2B1 are associated with various diseases, including multiple cancers and inclusion body myopathy.
免疫印迹
WB result of HNRNPA2B1 Mouse mAb
Primary antibody: HNRNPA2B1 Mouse mAb at 1/20000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: K532 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 36, 37 kDaWB result of HNRNPA2B1 Mouse mAb
Primary antibody: HNRNPA2B1 Mouse mAb at 1/20000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 37 kDaWB result of HNRNPA2B1 Mouse mAb
Primary antibody: HNRNPA2B1 Mouse mAb at 1/20000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 37 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-HNRNPA2B1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-HNRNPA2B1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-HNRNPA2B1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-HNRNPA2B1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-HNRNPA2B1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in NIH/3T3 cells. Anti-HNRNPA2B1 antibody was used at 1/800 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in C6 cells. Anti-HNRNPA2B1 antibody was used at 1/800 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in U-2 OS cells. Anti-HNRNPA2B1 antibody was used at 1/800 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of HNRNPA2B1 in tumor tissues.
Expression of HNRNPA2B1 in human tissues.
Expression of HNRNPA2B1 in mouse & rat tissues.
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