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Rabbit抗原名称
OTX2分子别名
Homeobox protein OTX2; Orthodenticle homolog 2免疫原
Synthetic Peptide细胞定位
NucleusAccession
P32243克隆号
S-2095-8抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
NTERA-2, normal mouse E14 foetal brain预测反应种属
(反应种属缩写表)Ys, Zf纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:2000 Hu, Ms IHC-P 1:5000 Ms IF 1:500 Ms
OTX2 protein, also known as orthodenticle homeobox 2, is a transcription factor encoded by the OTX2 gene and belongs to the homeobox-containing gene family. It plays crucial roles in various developmental processes, particularly in the formation and function of the central nervous system. OTX2 is highly expressed in the brain, including regions such as the cerebral cortex and cerebellum. This protein is also involved in the development of the retina, where it is expressed in photoreceptors and bipolar cells. Additionally, OTX2 has been implicated in non-cell-autonomous activities, meaning it can influence neighboring cells through diffusion and interaction. Abnormalities in the OTX2 gene have been associated with conditions like microphthalmia and pituitary hormone deficiency. Recent studies also suggest a potential link between OTX2 and major depressive disorders.
免疫印迹
WB result of OTX2 Recombinant Rabbit mAb
Primary antibody: OTX2 Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: NTERA-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 35 kDaWB result of OTX2 Recombinant Rabbit mAb
Primary antibody: OTX2 Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: normal mouse E14 foetal brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 35 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse E14.5 choroid plexus. Anti-OTX2 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse E14.5 retina. Anti-OTX2 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse E14.5 retina. Anti- OTX2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse E14.5 retina. Anti- OTX2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse E14.5 retina. Anti- OTX2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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