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宿主来源
Rabbit抗原名称
CXCR4分子别名
C-X-C chemokine receptor type 4; CXC-R4; CXCR-4; FB22; Fusin; HM89; LCR1; Leukocyte-derived seven transmembrane domain receptor (LESTR); CD184细胞定位
Lysosome, Endosome, Cell membraneAccession
P61073克隆号
S-5318抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, Jurkat, Raji, Ramos纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu IHC-P 1:800 Hu
CXCR4, also known as CD184 or fusin, is a 45 kD seven-transmembrane G-protein-linked CXC chemokine receptor. It is widely expressed on various human cells, including leukocytes, hematopoietic stem cells, epithelial cells, endothelial cells, and cancer cells. This receptor is specific for stromal-derived factor-1 (SDF-1, also called CXCL12) and plays a crucial role in cell migration, hematopoiesis, cell homing, and the proliferation of non-hematopoietic cells. Additionally, CXCR4 serves as a co-receptor for T-cell tropic HIV viruses, enabling their entry into human lymphocytes. In cancer, CXCR4 is involved in tumor cell survival, proliferation, migration, and metastasis, making it a potential therapeutic target.
免疫印迹
WB result of CXCR4 Recombinant Mouse mAb
Primary antibody: CXCR4 Recombinant Mouse mAb at 1/1000 dilution
Lane 1: unboiled HepG2 whole cell lysate 20 µg
Lane 2: unboiled HeLa whole cell lysate 20 µg
Lane 3: unboiled Jurkat whole cell lysate 20 µg
Lane 4: unboiled Raji whole cell lysate 20 µg
Lane 5: unboiled Ramos whole cell lysate 20 µg
Negative control: unboiled HepG2 whole cell lysate
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 38 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human spleen. Anti-CXCR4 antibody was used at 1/800 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CXCR4 antibody was used at 1/800 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CXCR4 antibody was used at 1/800 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CXCR4 in tumor tissues.
Expression of CXCR4 in human tissues.
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