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Rabbit抗原名称
CK1α分子别名
Casein kinase I isoform alpha; CKI-alpha; CK1; Casein kinase I isoform alpha-like; CKI-alpha-like免疫原
Synthetic Peptide细胞定位
Cytoskeleton, Nucleus, CytoplasmAccession
P48729, Q8N752克隆号
S-4407-18抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
MCF7, HeLa, Jurkat, HCT 116, U-87 MG, A549, RAW264.7, NIH/3T3, PC-12, rat brain预测反应种属
(反应种属缩写表)C.el, Ck, Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu, Ms, Rt
CK1α (casein kinase 1 isoform alpha) is a ubiquitously expressed, constitutively active serine/threonine kinase that docks to a conserved recognition motif (pSer/pThr-X-X-S/T) in hundreds of cytoplasmic, nuclear and membrane substrates, thereby governing Wnt/β-catenin signaling by phosphorylating β-catenin for β-TrCP-mediated degradation, synchronizing circadian rhythms through PER protein turnover, regulating DNA-damage checkpoints by modifying p53 and MDM2, driving hematopoietic stem-cell maintenance via AKT pathway modulation, and serving as an essential, druggable node whose loss-of-function triggers ribosomal stress and selective apoptosis in acute myeloid leukemia cells.
免疫印迹
WB result of CSNK1A1 + CSNK1A1L Recombinant Rabbit mAb
Primary antibody: CSNK1A1 + CSNK1A1L Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: HCT 116 whole cell lysate 20 µg
Lane 5: U-87 MG whole cell lysate 20 µg
Lane 6: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 34 kDaWB result of CSNK1A1 + CSNK1A1L Recombinant Rabbit mAb
Primary antibody: CSNK1A1 + CSNK1A1L Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW 264.7 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 34 kDaWB result of CSNK1A1 + CSNK1A1L Recombinant Rabbit mAb
Primary antibody: CSNK1A1 + CSNK1A1L Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 34 kDa
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