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Rabbit抗原名称
ClpX分子别名
ATP-dependent clpX-like chaperone, mitochondrial; ATP-dependent Clp protease ATP-binding subunit clpX-like; mitochondrial; Caseinolytic mitochondrial matrix peptidase chaperone subunit XImported; CLPX免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
O76031克隆号
S-4460-33抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Rt, Mk阳性样本
HT-1080, Saos-2, A673, HeLa, rat stomach, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Rt, Mk IHC-P 1:50 Hu
ClpX is a highly conserved ATP-dependent molecular chaperone belonging to the Clp/Hsp100 protein family, which plays a critical role in protein quality control across various biological systems, including bacteria, mitochondria, and chloroplasts. This protein typically exists as a homohexameric ring structure, with each monomer consisting of approximately 420–450 amino acids, containing an N-terminal zinc-binding domain (ZBD), a central AAA+ ATPase domain, and a C-terminal PDZ domain. The core function of ClpX lies in its ability to specifically recognize aberrant or misfolded proteins carrying SsrA tags, ssrA-like sequences, or specific C-terminal degradation signals, and using the energy generated from ATP hydrolysis, to unfold and translocate these substrate proteins into the proteolytic chamber of its associated ClpP protease for degradation. The ClpXP protease complex formed by ClpX and ClpP is not only responsible for clearing toxic protein aggregates within cells to maintain proteostasis, but also participates in regulating key biological processes such as the cell cycle, gene expression, and stress responses. In medical research, ClpX has been considered a potential target for antimicrobial and anti-tumor agents due to its essentiality in bacterial pathogens (such as Mycobacterium tuberculosis and Staphylococcus aureus) and its homologous function in eukaryotic mitochondria—for example, ADEP-class compounds targeting ClpX have emerged as a research hotspot in the development of novel antibiotics.
免疫印迹
WB result of ClpX Recombinant Rabbit mAb
Primary antibody: ClpX Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HT-1080 whole cell lysate 20 µg
Lane 2: Saos-2 whole cell lysate 20 µg
Lane 3: A673 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 62 kDaWB result of ClpX Recombinant Rabbit mAb
Primary antibody: ClpX Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat stomach lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 62 kDaWB result of ClpX Recombinant Rabbit mAb
Primary antibody: ClpX Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 62 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-ClpX antibody was used at 1/50 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-ClpX antibody was used at 1/50 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of ClpX in tumor tissues.
Expression of ClpX in human tissues.
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