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宿主来源
Mouse抗原名称
GAPDH分子别名
Glyceraldehyde-3-phosphate dehydrogenase; GAPD细胞定位
Cytoskeleton, Nucleus, CytoplasmAccession
P04406, P16858克隆号
S-4721抗体类型
Mouse mAb抗体同种型
IgG2b反应种属 ?
Hu, Ms, Rt, Bv, Rb, Dg, Gt, Zf, Ck, Mk阳性样本
HeLa, NIH/3T3, C6纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
ICC ?
稀释度
应用 稀释度 推荐种属 ICC 1:500 Hu, Ms, Rt ICFCM 1:2000 Hu
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ubiquitous and highly conserved multifunctional protein that plays a central role in cellular metabolism, primarily catalyzing the sixth step of glycolysis where it converts glyceraldehyde-3-phosphate into 1,3-bisphosphoglycerate while simultaneously reducing NAD+ to NADH. Beyond its canonical function in energy production, GAPDH exhibits remarkable "moonlighting" activities depending on its subcellular localization, participating in diverse processes such as DNA repair, transcriptional regulation, vesicular transport, and the initiation of apoptosis under stress conditions like oxidative damage or nitrosative stress. Due to its constitutive and relatively stable expression across various tissues and experimental conditions, GAPDH has historically been widely used as a loading control in techniques like Western blotting and RT-PCR, although recent studies caution against this practice without validation since its levels can fluctuate significantly in response to specific metabolic states or pathological conditions. Structurally, it typically functions as a homotetramer, and its involvement in several neurodegenerative diseases, including Huntington's and Alzheimer's, highlights its critical importance in maintaining cellular homeostasis and its potential as a therapeutic target.
流式分析
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling Alexa Fluor® 488 Mouse IgG2b Isotype Control (black line histogram) or SDT Alexa Fluor® 488 GAPDH Recombinant Mouse mAb (red line histogram) at 1:2000 dilution (0.1 μg), cells without incubation with primary antibody and secondary antibody (blue line histogram) was used as unlabeled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- GAPDH Recombinant Mouse mAb (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in NIH/3T3 cells. Anti- GAPDH Recombinant Mouse mAb (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in C6 cells. Anti- GAPDH Recombinant Mouse mAb (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
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