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Mouse抗原名称
FLAG tag分子别名
DYKDDDDK tag克隆号
S-5005抗体类型
Mouse mAb抗体同种型
IgG2b反应种属 ?
Species Independent纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:5000-1:50000 Species independent
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a peptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). It is one of the most specific tags and it is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells. FLAG-tag has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. FLAG-tag can also be used in the isolation of protein complexes with multiple subunits.
免疫印迹
WB result of FLAG Tag Recombinant Mouse mAb
Primary antibody: FLAG Tag Recombinant Mouse mAb at 1/20000 dilution
Lane 1: 293F transfected with empty vector whole cell lysate 20 µg
Lane 2: 293F transfected with N-FLAG fusion protein whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 22 kDa
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