TXNRD1 Mouse mAb (S-M0053)
Thioredoxin reductase 1,cytoplasmic,Gene associated with retinoic and interferon-induced mortality 12 protein (GRIM-12,Gene associated with retinoic and IFN-induced mortality 12 protein),KM-102-derived reductase-like factor,Peroxidase TXNRD1,Thioredoxin reductase TR1,GRIM12,KDRF
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宿主来源
Mouse抗原名称
TXNRD1分子别名
Thioredoxin reductase 1, cytoplasmic; Gene associated with retinoic and interferon-induced mortality 12 protein (GRIM-12; Gene associated with retinoic and IFN-induced mortality 12 protein); KM-102-derived reductase-like factor; Peroxidase TXNRD1; Thioredoxin reductase TR1; GRIM12; KDRF细胞定位
Cytoplasm, NucleusAccession
Q16881克隆号
S-M0053抗体类型
Mouse mAb抗体同种型
IgG1反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, A549, Jurkat, K562,纯化方式
Protein G浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:2000-1:10000 Hu, Ms, Rt IHC-P 1:500-1:2000 Hu, Ms, Rt ICC 1:200-1:800 Hu, Ms, Rt
TXNRD1, or cytosolic thioredoxin reductase 1, is a homodimeric flavoprotein of approximately 649 amino acids and 71 kDa, encoded by the TXNRD1 gene and belonging to the pyridine nucleotide-disulfide oxidoreductase family. It is a core component of the mammalian thioredoxin system. Each subunit of this protein contains an FAD cofactor and a rare amino acid—selenocysteine—located at the C-terminal active center, which is encoded by the UGA codon and requires precise translation through a SECIS element. Functionally, TXNRD1 utilizes NADPH as an electron donor to primarily reduce oxidized thioredoxin, while also directly reducing various substrates such as hydrogen peroxide, thereby playing a critical role in maintaining cellular redox homeostasis and regulating cell growth and differentiation. The protein is primarily localized in the cytosol, but alternative splicing generates different isoforms targeted to the nucleus or plasma membrane, further expanding its functional diversity. In addition to its classic antioxidant functions, recent studies have revealed non-enzymatic activities of TXNRD1, such as enhancing cGAS enzyme activity by binding to it in senescent cells, thereby driving the cGAS-STING inflammatory pathway to promote the senescence-associated secretory phenotype, thus playing an important role in the tumor microenvironment and inflammaging. Given its critical role in supporting proliferation and counteracting oxidative stress in cancer cells, TXNRD1 has emerged as a promising target for anticancer drug development.
免疫印迹
WB result of TXNRD1 Mouse mAb
Primary antibody: TXNRD1 Mouse mAb at 1/5000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 58 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-TXNRD1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-TXNRD1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-TXNRD1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-TXNRD1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-TXNRD1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of TXNRD1 in tumor tissues.
Expression of TXNRD1 in human tissues.
Expression of TXNRD1 in mouse & rat tissues.
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