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宿主来源
Mouse抗原名称
RAB27A分子别名
Ras-related protein Rab-27A; RAB27细胞定位
Lysosome, EndosomeAccession
P51159克隆号
S-M0050抗体类型
Mouse mAb抗体同种型
IgG1反应种属 ?
Hu, Ms阳性样本
HeLa, K562, Jurkat, HepG2, Mouse lung纯化方式
Protein G浓度
1.6 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu, Ms IHC-P 1:5000-1:10000 Hu, Ms ICFCM 1:6000 Hu, Ms
RAB27A is a small GTPase belonging to the Ras superfamily, encoded by the RAB27A gene, with a molecular weight of approximately 25 kDa. It is primarily localized to secretory vesicles and the plasma membrane within cells, where it functions as a key regulator of vesicular trafficking and exocytosis. RAB27A interacts with various downstream effector proteins in its GTP-bound active form, most notably through binding to synaptotagmin-like proteins (Slps, such as Slp2-a and Slp4-a) and Slac2 family members, thereby regulating vesicle maturation, docking, directed movement, and fusion with the plasma membrane. RAB27A plays important physiological roles in various secretory cell types, particularly in cytotoxic T lymphocytes, where it mediates the polarized secretion of cytolytic granules to enable immune killing functions. In melanocytes, RAB27A forms a ternary complex with myosin Va and melanophilin, which is essential for the transport of melanosomes to dendritic tips. Loss-of-function mutations in the RAB27A gene are the genetic cause of Griscelli syndrome type 2 (GS-2), a disorder characterized by partial albinism (due to impaired melanosome transport) and severe immunodeficiency (due to defective degranulation of cytotoxic T cells and NK cells), often accompanied by hemophagocytic lymphohistiocytosis. Furthermore, aberrant expression of RAB27A is closely associated with tumor invasion and metastasis in various cancers, making it a potential target for research in immunotherapy and tumor metastasis intervention.
免疫印迹
WB result of RAB27A Mouse mAb
Primary antibody: TRAB27A Mouse mAb at 1/5000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 26 kDaWB result of RAB27A Mouse mAb
Primary antibody: TRAB27A Mouse mAb at 1/5000 dilution
Lane 1: mouse lung lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 26 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human stomach. Anti-RAB27A antibody was used at 1/10000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-RAB27A antibody was used at 1/10000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-RAB27A antibody was used at 1/10000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-RAB27A antibody was used at 1/10000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of RAB27A in tumor tissues.
Expression of RAB27A in human tissues.
Expression of RAB27A in mouse & rat tissues.
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