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宿主来源
Rabbit抗原名称
SLFN5分子别名
Schlafen family member 5免疫原
Recombinant Protein细胞定位
NucleusAccession
Q08AF3抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu阳性样本
A549, T-47D, LNCaP, A431纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu ICC 1:500 Hu
SLFN5 (Schlafen family member 5) is a protein-coding gene that belongs to the Schlafen family, which is involved in various biological processes such as cell cycle regulation, immune response, and viral infection. SLFN5 has been identified as a regulator of cell motility and invasion in renal cell carcinoma, and its expression is associated with poor prognosis in colorectal adenocarcinoma. Additionally, SLFN5 acts as a restriction factor against herpes simplex virus type 1 (HSV-1) infection by suppressing viral transcription. It is also regulated by the androgen receptor in castration-resistant prostate cancer.
免疫印迹
WB result of SLFN5 Rabbit pAb
Primary antibody: SLFN5 Rabbit pAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: T-47D whole cell lysate 20 µg
Lane 3: LNCaP whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 101 kDa
Observed MW: 100 kDa
免疫细胞化学
ICC shows positive staining in A431 cells. Anti- SLFN5 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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