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Niemann Pick C1 Recombinant Rabbit mAb (S-5243)

NPC intracellular cholesterol transporter 1,Niemann-Pick C1 protein,NPC1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B60149
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Niemann Pick C1

  • 分子别名

    NPC intracellular cholesterol transporter 1; Niemann-Pick C1 protein; NPC1

  • 细胞定位

    Endosome, Lysosome

  • Accession

    O15118

  • 克隆号

    S-5243

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt, Mk

  • 阳性样本

    U-87 MG, HT-1080, SK-BR-3, PC-3, 3T3-L1, Neuro-2a, rat liver, COS-7

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000-1:2000 Hu, Ms, Rt, Mk
    IHC-P 1:250 Hu, Ms, Rt
    ICC 1:500 Hu, Ms
背景介绍

  • Niemann–Pick C1 (NPC1) is a large (~142 kDa) polytopic integral membrane glycoprotein that resides predominantly in the limiting membranes of late endosomes and lysosomes, where it functions as a sterol-sensing shuttle: its N-terminal “cholesterol-binding pocket” captures cholesterol (and other hydrophobic metabolites) from the luminal leaflet, a ring of 13 trans-membrane helices forms a hydrophobic conduit that is gated by cytosolic loop 6, and the C-terminal “NPC2-interacting” domain receives cholesterol from the soluble lysosomal protein NPC2, thereby enabling net export of cholesterol to the cytoplasmic leaflet for redistribution to other organelles; pathogenic mutations in NPC1 block this lipid efflux, causing the fatal neurovisceral disorder Niemann–Pick disease type C1 characterized by lysosomal lipid accumulation, neurodegeneration and premature death.

  • 免疫印迹

    • WB result of Niemann Pick C1 Recombinant Rabbit mAb
      Primary antibody: Niemann Pick C1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: unboiled U-87 MG whole cell lysate 20 µg
      Lane 2: unboiled HT-1080 whole cell lysate 20 µg
      Lane 3: unboiled SK-BR-3 whole cell lysate 20 µg
      Lane 4: unboiled PC-3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 142 kDa
      Observed MW: 150-250 kDa

    • WB result of Niemann Pick C1 Recombinant Rabbit mAb
      Primary antibody: Niemann Pick C1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: unboiled 3T3-L1 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 142 kDa
      Observed MW: 160-230 kDa

    • WB result of Niemann Pick C1 Recombinant Rabbit mAb
      Primary antibody: Niemann Pick C1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: unboiled rat liver lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 142 kDa
      Observed MW: 160-230 kDa

    • WB result of Niemann Pick C1 Recombinant Rabbit mAb
      Primary antibody: Niemann Pick C1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: unboiled COS-7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 142 kDa
      Observed MW: 150-230 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human kidney. Anti- Niemann Pick C1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti- Niemann Pick C1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse liver. Anti- Niemann Pick C1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat liver. Anti- Niemann Pick C1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HepG2 cells. Anti-Niemann Pick C1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    • ICC shows positive staining in Neuro-2a cells. Anti-Niemann Pick C1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of Niemann Pick C1 in tumor tissues.

    • Expression of Niemann Pick C1 in human tissues.

    • Expression of Niemann Pick C1 in mouse & rat tissues.

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