LILRB1 Recombinant Rabbit mAb (S-5001)
Leukocyte immunoglobulin-like receptor subfamily B member 1,LIR-1,Leukocyte immunoglobulin-like receptor 1,CD85 antigen-like family member J,Immunoglobulin-like transcript 2 (ILT-2),Monocyte/macrophage immunoglobulin-like receptor 7 (MIR-7),ILT2,LIR1,MIR7,LILRB1
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宿主来源
Rabbit抗原名称
CD85j分子别名
Leukocyte immunoglobulin-like receptor subfamily B member 1; LIR-1; Leukocyte immunoglobulin-like receptor 1; CD85 antigen-like family member J; Immunoglobulin-like transcript 2 (ILT-2); Monocyte/macrophage immunoglobulin-like receptor 7 (MIR-7); ILT2; LIR1; MIR7; LILRB1细胞定位
Cell membrane, SecretedAccession
Q8NHL6克隆号
S-5001抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Daudi, Raji纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:2000 Hu
CD85j, also known as LILRB1 or ILT2, is a 110 kDa transmembrane glycoprotein belonging to the leukocyte immunoglobulin-like receptor (LILR) family. It is an inhibitory receptor broadly expressed on leukocytes, including B cells, monocytes, subsets of NK cells, memory/effector CD8+ T cells, gamma δ T cells, and monocyte-derived dendritic cells. CD85j recognizes a wide range of classical HLA-class I allelic variants, as well as non-classical molecules such as HLA-F and HLA-G, by binding to the conserved α3 domain. Additionally, it binds to the human cytomegalovirus-encoded MHC class I homologue UL18. The engagement of CD85j by its ligands induces inhibitory signals that downregulate immune responses, including the inhibition of antigen-induced proliferation and activation of CD8+ T cells, CD4+ T cells, NK cells, and gamma δ T cells. On dendritic cells, ligation of CD85j inhibits the production of cytokines like IL-10, IL-12p70, and TGF-beta and protects from Fas-mediated apoptosis.
免疫印迹
WB result of LILRB1 Recombinant Rabbit mAb
Primary antibody: LILRB1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: Daudi whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Negative control: A431 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 110 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-LILRB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-LILRB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-LILRB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-LILRB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-LILRB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of LILRB1 in tumor tissues.
Expression of LILRB1 in human tissues.
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