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宿主来源
Rabbit抗原名称
KCNMA1分子别名
Calcium-activated potassium channel subunit alpha-1; BK channel; BKCA alpha; Calcium-activated potassium channel; subfamily M subunit alpha-1; K(VCA)alpha; KCa1.1; KCNMA; SLO免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
Q12791克隆号
S-2926-33抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
SH-SY5Y, Neuro-2a预测反应种属
(反应种属缩写表)Xe, Zf, Rb, Bv, Dg, Pg, Mq, Ck纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:2000 Hu, Ms, Rt
The α subunit encoded by the KCNMA1 gene is the core pore-forming protein that constitutes the large-conductance calcium-activated potassium channel (BK channel, also known as MaxiK or KCa1.1). This protein has a molecular weight of approximately 134 kDa and belongs to the voltage-gated potassium channel superfamily. Its unique feature lies in its ability to respond simultaneously to membrane depolarization and increases in intracellular calcium concentration, mediating potassium efflux to rapidly repolarize or hyperpolarize the cell membrane, thereby precisely regulating the excitability of various excitable cells, including neurons and smooth muscle cells. Structurally, four identical subunits of the KCNMA1 protein assemble into a homotetramer, with each subunit containing seven transmembrane segments (S0–S6) and a large intracellular C-terminal domain. The S4 segment serves as the voltage sensor, while the C-terminus contains two RCK domains harboring high-affinity calcium-binding sites known as the "calcium bowl" as well as magnesium-binding sites, both of which cooperatively mediate calcium/magnesium-dependent channel activation. Through this unique dual activation mechanism, KCNMA1 plays a central role in regulating vascular smooth muscle tone, controlling neurotransmitter release, maintaining auditory tuning in cochlear hair cells, and participating in innate immunity, among other critical physiological processes. Furthermore, the function of this protein is finely modulated by alternative splicing, phosphorylation, and other post-translational modifications, as well as by association with auxiliary β or γ subunits. Both loss-of-function and gain-of-function mutations in the KCNMA1 gene are closely associated with a spectrum of neurological disorders, including generalized epilepsy, paroxysmal dyskinesia, and ataxia.
免疫印迹
WB result of KCNMA1 Recombinant Rabbit mAb
Primary antibody: KCNMA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 137 kDa
Observed MW: 150 kDaWB result of KCNMA1 Recombinant Rabbit mAb
Primary antibody: KCNMA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 137 kDa
Observed MW: 150 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-KCNMA1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-KCNMA1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-KCNMA1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-KCNMA1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-KCNMA1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-KCNMA1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of KCNMA1 in tumor tissues.
Expression of KCNMA1 in human tissues.
Expression of KCNMA1 in mouse & rat tissues.
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