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Rabbit抗原名称
HTR2A分子别名
5-hydroxytryptamine receptor 2A; 5-HT-2; 5-HT-2AAlternative nameSerotonin receptor 2A; HTR2免疫原
Synthetic Peptide细胞定位
Cell membrane, SynapseAccession
P28223克隆号
S-4426-14抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
Raji, U-87, mouse heart, mouse cerebellum, mouse brain, rat heart, rat cerebellum, rat brain, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu, Ms, Rt, Mk ICC 1:500 Hu
HTR2A, also known as the 5-hydroxytryptamine receptor 2A, is a G protein-coupled receptor composed of 471 amino acids with a molecular weight of approximately 52 kDa. It serves as a key receptor for the neurotransmitter serotonin (5-hydroxytryptamine) in the brain and is widely distributed in the cerebral cortex and basal ganglia, as well as in smooth muscle cells of peripheral tissues. When activated by serotonin or psychoactive substances such as LSD and psilocybin, HTR2A primarily couples to the Gq protein, activating phospholipase C and triggering a cascade of intracellular calcium release and downstream signaling pathways, thereby modulating neural activity, perception, cognition, and emotion. Consequently, dysfunction of HTR2A is closely associated with various psychiatric disorders, including schizophrenia, depression, and substance dependence, making it an important therapeutic target for the development of antipsychotic and antidepressant drugs.
免疫印迹
WB result of HTR2A Recombinant Rabbit mAb
Primary antibody: HTR2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: U-87 MG whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDaWB result of HTR2A Recombinant Rabbit mAb
Primary antibody: HTR2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Lane 2: mouse cerebellum lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDaWB result of HTR2A Recombinant Rabbit mAb
Primary antibody: HTR2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Lane 2: rat cerebellum lysate 20 µg
Lane 3: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDaWB result of HTR2A Recombinant Rabbit mAb
Primary antibody: HTR2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDa
免疫细胞化学
ICC shows positive staining in U-87 MG cells. Anti-HTR2A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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