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GPT Rabbit Polyclonal Antibody

Alanine aminotransferase 1,ALT1,Glutamate pyruvate transaminase 1 (GPT 1),Glutamic--alanine transaminase 1,Glutamic--pyruvic transaminase 1,Gpt1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B60141
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    GPT

  • 分子别名

    Alanine aminotransferase 1; ALT1; Glutamate pyruvate transaminase 1 (GPT 1); Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; Gpt1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm

  • Accession

    Q8QZR5

  • 抗体类型

    Polyclonal antibody

  • 抗体同种型

    IgG

  • 反应种属 ?

    Ms, Rt

  • 阳性样本

    mouse liver, mouse heart, rat liver, rat heart

  • 纯化方式

    Immunogen Affinity

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Ms, Rt
背景介绍

  • GPT, also known as alanine aminotransferase 1 (encoded by the human GPT gene), is a key metabolic enzyme located in the cytoplasm. Its core function is to catalyze the reversible transamination reaction between alanine and α-ketoglutarate, producing pyruvate and glutamate, thereby playing an important role in the intermediary metabolism of glucose and amino acids. Although GPT is expressed in various tissues including the liver, heart, muscle, adipose tissue, and kidneys, it is relatively abundant in hepatocytes. Consequently, when liver cells are damaged due to drug toxicity, infection, alcohol, or fatty liver disease, large amounts of GPT are released into the bloodstream. Therefore, serum GPT activity levels are widely used as a conventional biomarker for assessing liver injury.

  • 免疫印迹

    • WB result of GPT Rabbit pAb
      Primary antibody: GPT Rabbit pAb at 1/1000 dilution
      Lane 1: mouse spleen lysate 20 µg
      Lane 2: mouse liver lysate 20 µg
      Lane 3: mouse heart lysate 20 µg
      Low expression control: mouse spleen lysate
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 46 kDa
      Observed MW: 50 kDa

    • WB result of GPT Rabbit pAb
      Primary antibody: GPT Rabbit pAb at 1/1000 dilution
      Lane 1: rat spleen lysate 20 µg
      Lane 2: rat liver lysate 20 µg
      Lane 3: rat heart lysate 20 µg
      Low expression control: rat spleen lysate
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 46 kDa
      Observed MW: 50 kDa

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