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宿主来源
Rabbit抗原名称
CDH3分子别名
Cadherin-3; CDHP免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P22223克隆号
S-3576-116抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:500 Hu
CDH3, also known as P-cadherin (Placental cadherin), is a classical cadherin family member encoded by the CDH3 gene, functioning as a calcium-dependent, single-pass transmembrane cell adhesion glycoprotein. Its precursor protein consists of 829 amino acids and is processed by proteolytic cleavage to form a mature protein of approximately 118 kDa. Structurally, it comprises five extracellular cadherin repeats (with the N-terminal EC1 domain being particularly critical for adhesion specificity) responsible for calcium-dependent homophilic adhesion, a transmembrane region, and a highly conserved intracellular domain that links to the actin cytoskeleton through interactions with β-catenin and other proteins. Under physiological conditions, CDH3 expression exhibits high spatiotemporal specificity, being predominantly expressed in the basal layer proliferative cells of certain epithelial tissues, and plays a critical role in embryonic development, hair follicle cycling, and maintenance of normal mammary gland structure. Functional abnormalities or gene mutations in CDH3 can lead to hereditary disorders such as hypotrichosis with juvenile macular dystrophy or ectodermal dysplasia-syndactyly-macular dystrophy syndrome. In tumor biology, the function of CDH3 exhibits significant context-dependent bidirectionality: on the one hand, in various malignancies including breast cancer, gastric cancer, and pancreatic cancer, CDH3 is often aberrantly overexpressed, driving malignant progression by promoting cell migration, invasion, and epithelial–mesenchymal transition, and is closely associated with poor patient prognosis; on the other hand, in certain cancer types or specific microenvironments, it may also play a tumor-suppressive role. Based on its tumor-specific expression, therapeutic strategies targeting CDH3, including monoclonal antibodies and antibody-drug conjugates, have demonstrated promising potential in preclinical and early-stage clinical trials.
免疫印迹
WB result of CDH3 Recombinant Rabbit mAb
Primary antibody: CDH3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: BxPC-3 whole cell lysate 20 µg
Lane 4: HCT 116 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 91 kDa
Observed MW: 125 kDaWB result of CDH3 Recombinant Rabbit mAb
Primary antibody: CDH3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse placenta lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 91 kDa
Observed MW: 125 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human breast. Anti-CDH3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CDH3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CDH3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CDH3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CDH3 in tumor tissues.
Expression of CDH3 in human tissues.
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