VDAC1 Recombinant Rabbit mAb (S-5314)
Non-selective voltage-gated ion channel VDAC1,Outer mitochondrial membrane protein porin 1,Plasmalemmal porin,Porin 31HL,Porin 31HM,Voltage-dependent anion-selective channel protein 1 (VDAC-1,hVDAC1),VDAC
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宿主来源
Rabbit抗原名称
VDAC1分子别名
Non-selective voltage-gated ion channel VDAC1; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Porin 31HL; Porin 31HM; Voltage-dependent anion-selective channel protein 1 (VDAC-1; hVDAC1); VDAC细胞定位
Cell membrane, Mitochondrion, Endoplasmic reticulumAccession
P21796克隆号
S-5314抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HEK-293, HeLa, A431, Jurkat, MCF7, COLO205, HL-60, NIH/3T3, C2C12, PC-12, C6, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:10000 Hu, Ms, Rt IHC-P 1:2000 Hu, Ms, Rt
VDAC1 (Voltage-Dependent Anion Channel 1) is a key channel protein highly expressed on the outer mitochondrial membrane of eukaryotic cells and is a major member of the VDAC family. Structurally, it consists of 19 β-strands that form a transmembrane β-barrel structure, whose internal pore size can switch between "open" and "closed" states in response to changes in membrane potential, thereby regulating the transmembrane transport of various ions (such as Cl⁻ and K⁺) and metabolites (such as ATP, ADP, and pyruvate), serving as the central gateway for material exchange between mitochondria and the cytoplasm. Functionally, VDAC1 is far more than a passive channel; it dynamically interacts with a variety of ligands, including hexokinase and Bcl-2 family proteins, and is deeply involved in cellular energy metabolism, calcium homeostasis maintenance, and early regulation of apoptosis. When bound to pro-apoptotic proteins such as Bax, it can induce outer mitochondrial membrane permeabilization, releasing cytochrome c to initiate apoptosis, whereas anti-apoptotic proteins inhibit this process by occluding VDAC1. Consequently, aberrant expression or dysfunction of VDAC1 is closely associated with various diseases, including neurodegenerative disorders such as Alzheimer's disease and Parkinson's disease, myocardial ischemia-reperfusion injury, and various cancers, making it a popular target in drug therapy and disease diagnosis research.
免疫印迹
WB result of VDAC1 Recombinant Rabbit mAb
Primary antibody: VDAC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Lane 3: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDaWB result of VDAC1 Recombinant Rabbit mAb
Primary antibody: VDAC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Lane 6: COLO205 whole cell lysate 20 µg
Lane 7: HL-60 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDaWB result of VDAC1 Recombinant Rabbit mAb
Primary antibody: VDAC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-VDAC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-VDAC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-VDAC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-VDAC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-VDAC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-VDAC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-VDAC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-VDAC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of VDAC1 in tumor tissues.
Expression of VDAC1 in human tissues.
Expression of VDAC1 in mouse & rat tissues.
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