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Rabbit抗原名称
TdT分子别名
DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase (Terminal transferase); TDT; DNTT免疫原
Recombinant Protein细胞定位
NucleusAccession
P04053抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Jurkat, Molt-4, mouse thymus, rat thymus纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt ICC 1:500 Hu ICFCM 1:50 Hu
Terminal deoxynucleotidyl transferase (TdT) is a unique DNA polymerase that plays a crucial role in the immune system and DNA repair. Unlike most DNA polymerases, TdT can add nucleotides to the 3′-hydroxyl end of a DNA strand in a template-independent manner. This enzyme is primarily expressed in immature B and T lymphocytes and is essential for V(D)J recombination, a process that generates the vast diversity of antigen receptors in the adaptive immune system. By adding non-templated nucleotides (N-regions) at the junctions between V, D, and J gene segments, TdT significantly increases the diversity of T-cell receptors (TCRs) and B-cell receptors (BCRs), enhancing the immune system's ability to recognize a wide range of antigens. Additionally, TdT is involved in DNA repair, particularly in the non-homologous end joining (NHEJ) pathway, where it helps repair DNA double-strand breaks. Its ability to function without a template also makes TdT a valuable tool in biotechnology and genetic research, such as in DNA sequencing and end labeling techniques.
免疫印迹
WB result of TdT Rabbit pAb
Primary antibody: TdT Rabbit pAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: Molt-4 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 58 kDaWB result of TdT Rabbit pAb
Primary antibody: TdT Rabbit pAb at 1/1000 dilution
Lane 1: mouse thymus lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 58 kDaWB result of TdT Rabbit pAb
Primary antibody: TdT Rabbit pAb at 1/1000 dilution
Lane 1: rat thymus lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 58 kDa
流式分析
Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte) labelling TdT antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
ICC shows positive staining in Jurkat cells. Anti- TdT antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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