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SMARCA3 Recombinant Rabbit mAb (S-3575-154)

DNA-dependent ATPase/E3 ubiquitin-protein ligase HLTF,DNA-binding protein/plasminogen activator inhibitor 1 regulator,HIP116,Helicase-like transcription factor (HLTF),RING finger protein 80,RING-type E3 ubiquitin transferase HLTF,HIP116A,RNF80,SNF2L3,ZBU1,HLTF

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B60125
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    SMARCA3

  • 分子别名

    DNA-dependent ATPase/E3 ubiquitin-protein ligase HLTF; DNA-binding protein/plasminogen activator inhibitor 1 regulator; HIP116; Helicase-like transcription factor (HLTF); RING finger protein 80; RING-type E3 ubiquitin transferase HLTF; HIP116A; RNF80; SNF2L3; ZBU1; HLTF

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    Q14527

  • 克隆号

    S-3575-154

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt, Mk

  • 阳性样本

    HCT 116, HeLa, K562, NIH/3T3, C6, COS-7

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt, Mk
    IHC-P 1:500 Hu, Ms, Rt
    ICC 1:100 Hu, Ms, Rt
背景介绍

  • SMARCA3, also known as HLTF (helicase-like transcription factor), is a member of the SWI/SNF chromatin remodeling family. However, unlike traditional remodeling complexes, it functions independently through its unique dual enzymatic activities—ATP-dependent helicase-like activity and E3 ubiquitin ligase activity. Its functions are multifaceted: in DNA replication and damage response, it catalyzes K63-linked polyubiquitination of PCNA protein, promoting replication fork reversal to repair damaged DNA; simultaneously, it also serves as a specific E3 ubiquitin ligase for histone H3K23, stabilizing chromatin structure by maintaining the repressive histone modification H3K9me3. SMARCA3 is widely expressed in various tissues, particularly in the nervous system where it binds to the p11/annexin A2 complex and participates in regulating behavioral responses to antidepressant drugs. Its dysregulated expression is closely associated with tumors: in colorectal cancer, approximately 40% of cases exhibit SMARCA3 silencing due to promoter hypermethylation of the HLTF gene, which is associated with chromatin opening, oncogene activation, and tumor progression, establishing its important role as a tumor suppressor.

  • 免疫印迹

    • WB result of SMARCA3 Recombinant Rabbit mAb
      Primary antibody: SMARCA3 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: COLO205 whole cell lysate 20 µg
      Lane 2: HCT 116 whole cell lysate 20 µg
      Lane 3: HeLa whole cell lysate 20 µg
      Lane 4: K562 whole cell lysate 20 µg
      Low expression control: COLO205 whole cell lysate
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 114 kDa
      Observed MW: 140 kDa

    • WB result of SMARCA3 Recombinant Rabbit mAb
      Primary antibody: SMARCA3 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 114 kDa
      Observed MW: 140 kDa

    • WB result of SMARCA3 Recombinant Rabbit mAb
      Primary antibody: SMARCA3 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 114 kDa
      Observed MW: 140 kDa

    • WB result of SMARCA3 Recombinant Rabbit mAb
      Primary antibody: SMARCA3 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: COS-7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 114 kDa
      Observed MW: 140 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human testis. Anti-SMARCA3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SMARCA3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SMARCA3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-SMARCA3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse testis. Anti-SMARCA3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat stomach. Anti-SMARCA3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in K562 cells. Anti- SMARCA3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    • ICC shows positive staining in NIH/3T3 cells. Anti- SMARCA3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    • ICC shows positive staining in C6 cells. Anti- SMARCA3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of SMARCA3 in tumor tissues.

    • Expression of SMARCA3 in human tissues.

    • Expression of SMARCA3 in mouse & rat tissues.

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