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宿主来源
Rabbit抗原名称
Rab3D分子别名
Ras-related protein Rab-3D; GOV; RAB16; RAB3D免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
O95716克隆号
S-4406-22抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
TT, HT-29, HL-60纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:2000 Hu
Rab3D is a member of the small GTPase Rab family, belonging to the Rab3 subfamily, and is primarily localized on the membrane of secretory vesicles in secretory cells, playing a critical role in regulating vesicle trafficking and exocytosis. Through interactions with upstream regulators such as GEFs and GAPs, this protein cycles between the GTP-bound active state and the GDP-bound inactive state, thereby precisely controlling vesicle maturation, mobilization, docking, and fusion with target membranes. Rab3D is expressed in various tissues and is particularly abundant in pancreatic acinar cells and adipocytes, where its function is associated with the regulation of insulin-like growth factors and metabolic balance; it also participates in regulating neurotransmitter release, gastric acid secretion, and salivary secretion, making it an indispensable regulator of vesicle trafficking in exocrine and endocrine cells. Dysfunction or genetic deficiency of Rab3D is associated with multiple diseases; for example, Rab3D knockout mice exhibit increased bone mass and accelerated bone formation phenotypes, while downregulation of Rab3D expression in humans may be closely related to osteoporosis, obesity, and certain metabolic diseases, positioning it as an important molecular target for studying vesicle trafficking mechanisms and related metabolic disorders.
免疫印迹
WB result of Rab3D Recombinant Rabbit mAb
Primary antibody: Rab3D Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: TT whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: HL-60 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 24 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-Rab3D antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Rab3D antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Rab3D antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-Rab3D antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Rab3D in tumor tissues.
Expression of Rab3D in human & rat tissues.
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