热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 抗体 其他一抗

所有图片（1/13）

PODXL Recombinant Rabbit mAb (S-2014-9)

Podocalyxin,GCTM-2 antigen,Gp200,Podocalyxin-like protein 1 (PC,PCLP-1),PCLP,PCLP1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6011
规格
数量
收藏 分享
产品介绍

产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    PODXL

  • 分子别名

    Podocalyxin; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1 (PC; PCLP-1); PCLP; PCLP1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cell membrane

  • Accession

    O00592

  • 克隆号

    S-2014-9

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HT-29, HeLa, Caco-2, mouse kidney

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms
    IHC-P 1:5000 Hu, Ms, Rt
背景介绍

  • Podocalyxin-like protein 1 (PODXL) is a type I transmembrane protein belonging to the CD34 family of sialomucins, typically expressed in kidney podocytes, vascular endothelial cells, hematopoietic progenitors, mesothelium, and certain neurons. It has a highly glycosylated extracellular domain, a single transmembrane domain, and a short cytoplasmic tail. PODXL functions primarily as an anti-adhesive molecule, regulating cell morphology and adhesion, and is crucial for kidney development and function. In cancer, PODXL is often overexpressed and associated with aggressive tumor phenotypes, poor prognosis, and potentially chemoresistance.

  • 免疫印迹

    • WB result of PODXL Recombinant Rabbit mAb
      Primary antibody: PODXL Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: A-204 whole cell lysate 20 µg
      Lane 2: HT-29 whole cell lysate 20 µg
      Lane 3: HeLa whole cell lysate 20 µg
      Lane 4: Caco-2 whole cell lysate 20 µg
      Negative control: A204 whole cell lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 58 kDa
      Observed MW: 160-250 kDa

    • WB result of PODXL Recombinant Rabbit mAb
      Primary antibody: PODXL Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse kidney lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 58 kDa
      Observed MW: 150-170 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-PODXL antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-PODXL antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-PODXL antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human stomach. Anti-PODXL antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PODXL antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-PODXL antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse stomach. Anti-PODXL antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat colon. Anti-PODXL antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of PODXL in tumor tissue.

    • Expression of PODXL in human tissue.

    • Expression of PODXL in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

引用文献(1)

评论(0)