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Rabbit抗原名称
ALPP/ALPPL2分子别名
Alkaline phosphatase, placental type; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1 (PLAP-1); PLAP免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P05187克隆号
S-2089-36抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
A431纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:1000 Hu
ALPP/ALPPL2 proteins are members of the alkaline phosphatase family, which are closely related and regulated GPI-anchored proteins. ALPP, also known as placental alkaline phosphatase, and ALPPL2, also known as germ cell alkaline phosphatase or placental-like alkaline phosphatase 2, share a high degree of amino acid sequence similarity (98% homology) and have a highly restricted tissue expression pattern, primarily found in placental trophoblasts and certain tumor tissues. ALPPL2 is expressed in various cancers, including mesothelioma, ovarian cancer, and gastric cancer, and is considered a tumor-specific antigen due to its high tissue specificity. This makes it a promising target for cancer therapies, such as antibody-drug conjugates and CAR-T cell therapies.
免疫印迹
WB result of ALPP/ALPPL2 Recombinant Rabbit mAb
Primary antibody: ALPP/ALPPL2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 70 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human placenta. Anti-ALPP/ALPPL2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human small intestine. Anti-ALPP/ALPPL2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-ALPP/ALPPL2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-ALPP/ALPPL2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human seminoma. Anti-ALPP/ALPPL2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti-ALPP/ALPPL2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of ALPP/ALPPL2 in tumor tissue.
Expression of ALPP/ALPPL2 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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