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INI-1/SMARCB1 Recombinant Rabbit mAb (S-4068-21)

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1,BRG1-associated factor 47 (BAF47),Integrase interactor 1 protein,SNF5 homolog (hSNF5),BAF47,INI1,SNF5L1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B60108
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    INI-1/SMARCB1

  • 分子别名

    SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; BRG1-associated factor 47 (BAF47); Integrase interactor 1 protein; SNF5 homolog (hSNF5); BAF47; INI1; SNF5L1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    Q12824

  • 克隆号

    S-4068-21

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt, Mk

  • 阳性样本

    K562, HeLa, Jurkat, 293T, Daudi, NIH/3T3, Neuro-2a, RAW264.7, mouse brain, mouse spleen, PC-12, rat brain, rat spleen, COS-7

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt, Mk
    IHC-P 1:2000 Hu, Ms, Rt
    ICC 1:500 Hu
背景介绍

  • SMARCB1, also known as INI1 or BAF47, is a core component of the human SWI/SNF chromatin remodeling complex, which regulates gene transcriptional activity by altering chromatin structure. As a 47-kilodalton protein composed of 385 amino acids and localized in the nucleus, SMARCB1 can bind to DNA and plays critical roles in various life processes, including cell proliferation, differentiation, and tumor suppression. The protein is best known for its function as a tumor suppressor, and mutations or loss of its expression are closely associated with the development of multiple aggressive tumors. For instance, biallelic inactivation of SMARCB1 is a key molecular event leading to rhabdoid tumors—highly malignant neoplasms that predominantly affect infants and young children—and its germline mutations also underlie the rhabdoid tumor predisposition syndrome (RTPS1). Furthermore, variations in the SMARCB1 gene have also been linked to schwannomatosis (a benign nerve sheath tumor disorder) and Coffin-Siris syndrome (a hereditary developmental disorder), demonstrating the diversity of disease spectrums resulting from its functional abnormalities.

  • 免疫印迹

    • WB result of INI-1/SMARCB1 Recombinant Rabbit mAb
      Primary antibody: INI-1/SMARCB1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: A204 whole cell lysate 20 µg
      Lane 2: K562 whole cell lysate 20 µg
      Lane 3: HeLa whole cell lysate 20 µg
      Lane 4: Jurkat whole cell lysate 20 µg
      Lane 5: 293T whole cell lysate 20 µg
      Lane 6: Daudi whole cell lysate 20 µg
      Low expression control: A204 whole cell lysate
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 44 kDa
      Observed MW: 40 kDa

    • WB result of INI-1/SMARCB1 Recombinant Rabbit mAb
      Primary antibody: INI-1/SMARCB1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: Neuro-2a whole cell lysate 20 µg
      Lane 3: RAW264.7 whole cell lysate 20 µg
      Lane 4: mouse brain lysate 20 µg
      Lane 5: mouse spleen lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 44 kDa
      Observed MW: 40 kDa

    • WB result of INI-1/SMARCB1 Recombinant Rabbit mAb
      Primary antibody: INI-1/SMARCB1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: PC-12 whole cell lysate 20 µg
      Lane 2: rat brain lysate 20 µg
      Lane 3: rat spleen lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 44 kDa
      Observed MW: 44 kDa

    • WB result of INI-1/SMARCB1 Recombinant Rabbit mAb
      Primary antibody: INI-1/SMARCB1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: COS-7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 44 kDa
      Observed MW: 44 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-INI-1/SMARCB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human stomach. Anti-INI-1/SMARCB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-INI-1/SMARCB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-INI-1/SMARCB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung cancer. Anti-INI-1/SMARCB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-INI-1/SMARCB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-INI-1/SMARCB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat kidney. Anti-INI-1/SMARCB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- INI-1/SMARCB1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of INI-1/SMARCB1 in tumor tissues.

    • Expression of INI-1/SMARCB1 in human tissues.

    • Expression of INI-1/SMARCB1 in mouse & rat tissues.

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