分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
HNRNPL分子别名
Heterogeneous nuclear ribonucleoprotein L; HNRPL免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
P14866抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
Ramos, PANC-1, HT-1080, HepG2, PC-3, HeLa, RAW264.7, C6, COS-7纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:250 Hu, Ms, Rt ICC 1:500 Hu
HNRNPL, also known as heterogeneous nuclear ribonucleoprotein L, is a ubiquitously expressed RNA-binding protein in cells that primarily participates in pre-mRNA processing, packaging, and alternative splicing regulation. As a core component of the hnRNP complex, it is typically localized in the nucleoplasm, preferentially binds to CA-rich sequences on RNA, and can act as either an activator or inhibitor of exon inclusion, playing a critical role in gene expression regulation. HNRNPL contains multiple RNA recognition motifs (RRMs), which constitute the structural basis for its RNA-binding activity, and its functions are not confined to the nucleus; due to its ability to shuttle between the nucleus and cytoplasm, it may also participate in the regulation of mRNA stability in the cytoplasm. Increasing research has also revealed that HNRNPL, through interactions with molecules such as long non-coding RNAs (lncRNAs), plays important roles in the development, progression, and chemoresistance of various cancers (including bladder cancer, esophageal cancer, ovarian cancer, and colorectal cancer), making it a potential disease biomarker and therapeutic target.
免疫印迹
WB result of HNRNPL Rabbit pAb
Primary antibody: HNRNPL Rabbit pAb at 1/1000 dilution
Lane 1: Ramos whole cell lysate 20 µg
Lane 2: PANC-1 whole cell lysate 20 µg
Lane 3: HT-1080 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: PC-3 whole cell lysate 20 µg
Lane 6: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 64 kDaWB result of HNRNPL Rabbit pAb
Primary antibody: HNRNPL Rabbit pAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 64 kDaWB result of HNRNPL Rabbit pAb
Primary antibody: HNRNPL Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 64 kDaWB result of HNRNPL Rabbit pAb
Primary antibody: HNRNPL Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 70 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-HNRNPL antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-HNRNPL antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-HNRNPL antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-HNRNPL antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HepG2 cells. Anti-HNRNPL antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of HNRNPL in tumor tissues.
Expression of HNRNPL in human tissues.
Expression of HNRNPL in mouse & rat tissues.
评论(0)