HLA-ABC Recombinant Rabbit mAb (S-3995-2)
HLA class I histocompatibility antigen,A alpha chain,Human leukocyte antigen A (HLA-A),HLAA,HLA-A,B alpha chain,Human leukocyte antigen B (HLA-B),HLA-B,HLAB,C alpha chain,HLA-C,HLA-Cw,Human leukocyte antigen C,HLAC
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宿主来源
Rabbit抗原名称
HLA-ABC分子别名
HLA class I histocompatibility antigen, A alpha chain; Human leukocyte antigen A (HLA-A); HLAA; HLA-A; HLA class I histocompatibility antigen, B alpha chain; Human leukocyte antigen B (HLA-B); HLA-B; HLAB; HLA class I histocompatibility antigen, C alpha chain; HLA-C; HLA-Cw; Human leukocyte antigen C; HLA-C; HLAC免疫原
Recombinant Protein细胞定位
Cell membrane, Endoplasmic reticulum membraneAccession
P04439, P01889, P10321克隆号
S-3995-2抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Jurkat, HepG2, THP-1, Raji, Ramos纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu IHC-P 1:2000 Hu
HLA-ABC (also termed HLA class I classical heavy chains A, B, and C) are 44 kDa type I transmembrane glycoproteins encoded within the major histocompatibility complex on chromosome 6p21.3; each non-covalently associates with the invariant light chain β2-microglobulin (12 kDa) to form a peptide-binding heterodimer that is constitutively expressed on nearly all nucleated cells. These molecules serve as the principal conduit for presenting endogenously derived 8–11-mer peptides to CD8⁺ cytotoxic T lymphocytes, thereby orchestrating immune surveillance against intracellular pathogens, tumors, and transplanted tissues. Polymorphic residues concentrated in the α1 and α2 domains create an extraordinary diversity (thousands of alleles catalogued in the IPD-IMGT/HLA database), which underpins population-level resistance to infection yet constitutes the primary barrier to solid-organ and hematopoietic stem-cell transplantation through allorecognition. Beyond antigen presentation, HLA-ABC interacts with NK-cell inhibitory receptors (KIR and LILRB1) to calibrate natural killer cell responses, and its surface levels are dynamically modulated by cytokines such as interferon-γ, viral immune evasion proteins (e.g., HIV Nef, CMV US2/US11), and oncogenic signaling pathways, making quantitative HLA-ABC expression a prognostic biomarker in cancer immunotherapy.
免疫印迹
WB result of HLA-ABC Recombinant Rabbit mAb
Primary antibody: HLA-ABC Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: THP-1 whole cell lysate 20 µg
Lane 5: Raji whole cell lysate 20 µg
Lane 6: Ramos whole cell lysate 20 µg
Negative control: K562 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 40 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-HLA-ABC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-HLA-ABC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-HLA-ABC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-HLA-ABC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-HLA-ABC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of HLA-ABC in tumor tissues.
Expression of HLA-ABC in human tissues.
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