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Rabbit抗原名称
Dctd分子别名
Deoxycytidylate deaminase; DCTD免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P32321克隆号
S-4313-16抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
MCF7, BxPC-3, SK-OV-3, U-87 MG, HT-1080纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu IHC-P 1:200 Hu
DCTD, also known as dCMP deaminase, is encoded by the human DCTD gene and is a homotypic hexameric enzyme that plays a critical role in pyrimidine nucleotide metabolism. This protein is approximately 178 amino acids in length with a molecular weight of about 20 kDa, is primarily localized in the cytoplasm, and its enzymatic activity depends on zinc ions as a cofactor. Functionally, DCTD catalyzes the hydrolytic deamination of deoxycytidylate (dCMP) to convert it into deoxyuridylate (dUMP), which is the direct substrate for thymidylate synthase in the synthesis of dTMP. Therefore, DCTD provides the essential precursors for DNA replication and repair through this key step. The activity of this enzyme is tightly regulated by allosteric mechanisms, with its activator dCTP enhancing its activity while the end product dTTP inhibits it, thereby precisely maintaining the balance of the intracellular deoxynucleotide pool. In addition, the DCTD protein undergoes various post-translational modifications, including phosphorylation, ubiquitination, and acetylation, which may participate in the fine-tuning of its activity and function.
免疫印迹
WB result of Dctd Recombinant Rabbit mAb
Primary antibody: Dctd Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: BxPC-3 whole cell lysate 20 µg
Lane 3: SK-OV-3 whole cell lysate 20 µg
Lane 4: U-87 MG whole cell lysate 20 µg
Lane 5: HT-1080 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 24 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Dctd antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Dctd antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-Dctd antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Dctd in tumor tissues.
Expression of Dctd in human tissues.
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