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Rabbit抗原名称
FASN分子别名
Fatty acid synthase; FAS免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P49327克隆号
S-682-32抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 555性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICC ?
IF ?
稀释度
应用 稀释度 推荐种属 ICC 1:500 Hu IF 1:500 Hu, Ms, Rt
Fatty acid synthase is a multi-enzyme protein that catalyzes fatty acid synthesis. It is not a single enzyme but a whole enzymatic system composed of two identical 272 kDa multifunctional polypeptides, in which substrates are handed from one functional domain to the next. Its main function is to catalyze the synthesis of palmitate (C16:0, a long-chain saturated fatty acid) from acetyl-CoA and malonyl-CoA, in the presence of NADPH. FAS is upregulated in breast and gastric cancers, as well as being an indicator of poor prognosis, and so may be worthwhile as a chemotherapeutic target. FAS may also be involved in the production of an endogenous ligand for the nuclear receptor PPAR alpha, the target of the fibrate drugs for hyperlipidemia, and is being investigated as a possible drug target for treating the metabolic syndrome.
免疫细胞化学
ICC shows positive staining in A549 cells. Anti- FASN (Alexa Fluor® 555 Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
免疫荧光
IF shows positive staining in paraffin-embedded human colon cancer. Anti- FASN (Alexa Fluor® 555 Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human breast cancer. Anti- FASN (Alexa Fluor® 555 Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse liver. Anti- FASN (Alexa Fluor® 555 Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat liver. Anti- FASN (Alexa Fluor® 555 Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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