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宿主来源
Rabbit抗原名称
DPP4分子别名
Dipeptidyl peptidase 4; ADABP; Adenosine deaminase complexing protein 2 (ADCP-2); Dipeptidyl peptidase IV (DPP IV); T-cell activation antigen CD26; TP103; CD26; ADCP2免疫原
Synthetic Peptide细胞定位
Cell projection, Cell membraneAccession
P27487克隆号
S-1631-130抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Caco-2, LoVo, HT-29纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:1000 Hu
Dipeptidyl peptidase 4 (DPP4), also known as CD26, is a multifunctional serine protease widely expressed on the surface of various cells, including epithelial, endothelial, immune, and pancreatic cells. It is a type II transmembrane glycoprotein that can also exist in a soluble form in body fluids. DPP4 plays a crucial role in regulating the bioactivity of numerous peptides by cleaving N-terminal dipeptides, particularly those with proline or alanine in the penultimate position. This enzyme is best known for its involvement in glucose homeostasis through the degradation of incretin hormones like glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP), which are essential for postprandial insulin secretion. Inhibition of DPP4 has thus become a therapeutic target for type 2 diabetes, with DPP4 inhibitors being widely used as antihyperglycemic agents. Beyond glucose metabolism, DPP4 is implicated in various physiological and pathological processes, such as inflammation, immune function, and cancer progression. Additionally, elevated levels of circulating DPP4 have been associated with obesity and cardiovascular diseases, suggesting its potential role in linking metabolic disorders and vascular dysfunction.
免疫印迹
WB result of DPP4 Recombinant Rabbit mAb
Primary antibody: DPP4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SW480 whole cell lysate 20 µg
Lane 2: Caco-2 whole cell lysate 20 µg
Lane 3: LoVo whole cell lysate 20 µg
Lane 4: HT-29 whole cell lysate 20 µg
Negative control: SW480 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 90, 120 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-DPP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-DPP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-DPP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-DPP4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of DPP4 in tumor tissue.
Expression of DPP4 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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