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宿主来源
Rabbit抗原名称
TriMethyl-Histone H3 (K36)分子别名
TriMethyl-Histone H3-K36; H3K36me3细胞定位
NucleusAccession
P68431克隆号
S-M0025抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, NIH/3T3, C6纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ChIP
IHC-P ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IP 1:50 Hu, Ms, Rt IHC-P 1:2000 Hu, Ms, Rt ChIP 1:20-1:50 Hu, Ms, Rt
TriMethyl-Histone H3 (K36) refers to the trimethylation modification at the 36th lysine residue of histone H3, a highly conserved epigenetic mark primarily catalyzed by the SETD2 methyltransferase. It is widely distributed throughout the transcribed regions of actively transcribed genes (from downstream of the transcription start site to the gene end) and plays critical regulatory roles in transcriptional elongation, RNA splicing, and DNA damage repair. During transcription, phosphorylated RNA polymerase II recruits SETD2, which progressively catalyzes the deposition of H3K36me3 on nucleosomes. This modification subsequently regulates transcriptional elongation rates, suppresses cryptic promoter initiation, and promotes exon splicing through the recognition of effector proteins such as PSIP1/LEDGF and ZMYND11. In the field of DNA damage repair, H3K36me3 mediates efficient homologous recombination repair and mismatch repair by recruiting factors including the MSH6 mismatch repair complex and KDM4A/B. Reduced H3K36me3 levels or aberrant distribution are closely associated with various diseases, such as SETD2 mutation-driven cancers (including renal cancer and leukemia), as well as developmental abnormality syndromes caused by dysfunction of KMT3C/KMT3B (e.g., Lupski syndrome and Sotos-like phenotypes).
免疫印迹
WB result of TriMethyl-Histone H3 (K36) Recombinant Rabbit mAb
Primary antibody: TriMethyl-Histone H3 (K36) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrateWB result of TriMethyl-Histone H3 (K36) Recombinant Rabbit mAb
Primary antibody: TriMethyl-Histone H3 (K36) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrateWB result of TriMethyl-Histone H3 (K36) Recombinant Rabbit mAb
Primary antibody: TriMethyl-Histone H3 (K36) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human placenta. Anti-TriMethyl-Histone H3 (K36) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-TriMethyl-Histone H3 (K36) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-TriMethyl-Histone H3 (K36) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-TriMethyl-Histone H3 (K36) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of TriMethyl-Histone H3 (K36) in tumor tissues.
Expression of TriMethyl-Histone H3 (K36) in human tissues.
Expression of TriMethyl-Histone H3 (K36) in mouse & rat tissues.
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