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Rabbit抗原名称
TOM20分子别名
Mitochondrial import receptor subunit TOM20 homolog; Mitochondrial 20 kDa outer membrane protein; Outer mitochondrial membrane receptor Tom20; KIAA0016; TOMM20细胞定位
MitochondrionAccession
Q15388克隆号
S-M0015抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, MCF7, THP-1, HEK-293, HepG2, Raji, A549, NIH/3T3, mouse kidney, mouse liver, mouse brain, PC-12, rat kidney, rat liver, rat thymus纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:5000-1:10000 Hu, Ms, Rt IP 1:50 Hu, Ms, Rt IHC-P 1:5000 Hu, Ms, Rt ICC 1:500 Hu, Ms, Rt
TOM20 (Translocase of Outer Mitochondrial Membrane 20) is a ~20 kDa receptor subunit of the TOM complex embedded in the outer mitochondrial membrane; together with TOMM22 it forms the initial binding site that recognizes N-terminal matrix-targeting sequences on cytosol-synthesized pre-proteins and threads them through the TOMM40 channel into mitochondria, thereby governing mitochondrial biogenesis and bioenergetics, while also moonlighting as a mitophagy adaptor via PINK1, a regulator of apoptosis through BCL2/BAX, and a chaperone-like stabilizer of the androgen receptor in prostate cancer, making it a widely used marker for mitochondrial mass and a potential biomarker for diseases such as Parkinson’s and cancer .
免疫印迹
WB result of TOM20 Recombinant Rabbit mAb
Primary antibody: TOM20 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7whole cell lysate 20 µg
Lane 3: THP-1 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Lane 5: HepG2 whole cell lysate 20 µg
Lane 6: Raji whole cell lysate 20 µg
Lane 7: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 16 kDaWB result of TOM20 Recombinant Rabbit mAb
Primary antibody: TOM20 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Lane 3: mouse liver lysate 20 µg
Lane 4: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 16 kDaWB result of TOM20 Recombinant Rabbit mAb
Primary antibody: TOM20 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Lane 3: rat liver lysate 20 µg
Lane 4: rat thymus lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 16 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human heart. Anti-TOM20 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-TOM20 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-TOM20 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-TOM20 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach cancer. Anti-TOM20 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-TOM20 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-TOM20 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-TOM20 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-TOM20 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-TOM20 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in PC-12 cells. Anti-TOM20 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of TOM20 in tumor tissues.
Expression of TOM20 in human tissues.
Expression of TOM20 in mouse & rat tissues.
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