分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
NOVA1分子别名
RNA-binding protein Nova-1; Neuro-oncological ventral antigen 1; Ventral neuron-specific protein 1; Nova1免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q9JKN6克隆号
S-4031-71抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
mouse brain, PC-12纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:250 Ms, Rt ICC 1:500 Rt IF 1:500 Ms
NOVA1 (neuro-oncological ventral antigen 1) is a neuron-specific splicing factor characterized as an RNA-binding protein, belonging to a protein family that contains three KH domains, and is highly expressed primarily in the brain and nervous system. It specifically recognizes YCAY motifs (where Y represents a pyrimidine) on target RNAs and coordinates with other splicing factors to regulate the alternative splicing of numerous key neuronal genes, including those involved in synaptic transmission, axon guidance, and neuronal differentiation. NOVA1 plays a central role in nervous system development, synaptic plasticity maintenance, and neural circuit formation, and its dysfunction is closely associated with tumors of the central nervous system, such as paraneoplastic opsoclonus-myoclonus-ataxia syndrome. Gene knockout mouse experiments have shown that NOVA1 deficiency leads to abnormal neuronal migration, synaptic dysfunction, and severe behavioral abnormalities, including seizures and developmental delay. Furthermore, recent studies have revealed that NOVA1 influences addictive behaviors (such as the rewarding effects of morphine) and the risk of autism spectrum disorder by regulating the alternative splicing of specific genes, further highlighting its critical role in neuropathology.
免疫印迹
WB result of NOVA1 Recombinant Rabbit mAb
Primary antibody: NOVA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDa
This blot was developed with high sensitivity substrateWB result of NOVA1 Recombinant Rabbit mAb
Primary antibody: NOVA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-NOVA1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-NOVA1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in PC-12 cells. Anti-NOVA1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-NOVA1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of NOVA1 in mouse & rat tissues.
评论(0)