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GPR3 Recombinant Rabbit mAb (S-4112-61)

G-protein coupled receptor 3,ACCA

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B60077
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    GPR3

  • 分子别名

    G-protein coupled receptor 3; ACCA

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cell membrane

  • Accession

    P46089

  • 克隆号

    S-4112-61

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Mk

  • 阳性样本

    A204, U-87 MG, U-2 OS, SK-OV-3, HeLa, Raji, Neruo-2a, COS-7

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Mk
背景介绍

  • GPR3 (G protein-coupled receptor 3) is an orphan GPCR primarily expressed in the mammalian brain and oocytes, belonging to the Class A family. It is characterized by exceptionally high constitutive Gs protein activity, continuously activating adenylyl cyclase and elevating intracellular cAMP levels in the absence of exogenous ligands. Functionally, GPR3 promotes neuronal synapse growth, axon polarization, and anti-apoptosis through signaling pathways such as cAMP/PKA and PI3K/Akt, exerting important neuroprotective effects. Meanwhile, it maintains meiotic arrest in oocytes until the luteinizing hormone surge occurs. Recent studies have identified oleoylethanolamide (OEA) as its endogenous agonist, elucidated its cryo-EM structure in complex with G proteins, and revealed a regulatory mechanism involving dimerization-mediated autoinhibition. Dysfunction of GPR3 is closely associated with Alzheimer's disease (by modulating γ-secretase to promote Aβ generation), neuropathic pain, and premature ovarian failure, making it an important potential drug target for various diseases.

  • 免疫印迹

    • WB result of GPR3 Recombinant Rabbit mAb
      Primary antibody: GPR3 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: unboiled A204 whole cell lysate 20 µg
      Lane 2: unboiled U-87 MG whole cell lysate 20 µg
      Lane 3: unboiled U-2 OS whole cell lysate 20 µg
      Lane 4: unboiled SK-OV-3 whole cell lysate 20 µg
      Lane 5: unboiled HeLa whole cell lysate 20 µg
      Lane 6: unboiled Raji whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 35 kDa
      Observed MW: 42 kDa
      This blot was developed with high sensitivity substrate

    • WB result of GPR3 Recombinant Rabbit mAb
      Primary antibody: GPR3 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: unboiled Neuro-2a whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 35 kDa
      Observed MW: 42 kDa
      This blot was developed with high sensitivity substrate

    • WB result of GPR3 Recombinant Rabbit mAb
      Primary antibody: GPR3 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: unboiled COS-7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 35 kDa
      Observed MW: 42 kDa
      This blot was developed with high sensitivity substrate

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