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Rabbit抗原名称
Glucocorticoid Receptor分子别名
GRL; NR3C1细胞定位
Cytoplasm, NucleusAccession
P04150克隆号
S-M0030抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HepG2, HeLa, A549, HT-1080, U-87 MG, mouse liver, mouse kidney, rat liver, C6, COS-7纯化方式
Protein A浓度
0.25 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:500-1:2000 Hu, Ms, Rt, Mk IP 1:50 Hu, Ms, Rt, Mk
Glucocorticoid Receptor (GR) is a member of the nuclear receptor superfamily encoded by the NR3C1 gene, existing in several splice variants, among which GRα is the major functional isoform. In the absence of ligand, GR is primarily localized in the cytoplasm and forms a complex with molecular chaperones such as heat shock proteins to maintain its inactive conformation. Upon binding of glucocorticoids (e.g., cortisol or dexamethasone), GR undergoes a conformational change, dissociates from chaperone proteins, and rapidly translocates to the nucleus. Once in the nucleus, GR acts through two main mechanisms: directly binding to glucocorticoid response elements (GREs) in DNA to activate or repress target gene transcription (i.e., transactivation and transrepression), and modulating the expression of inflammation- and immune-related genes via protein-protein interactions with other transcription factors such as NF-κB and AP-1. This dual regulatory mechanism positions GR as a core regulator of the stress response, metabolic regulation (including gluconeogenesis and lipolysis), and anti-inflammatory actions. Dysregulation of GR signaling is closely linked to the pathogenesis of Cushing's syndrome, glucocorticoid resistance, and various autoimmune diseases, making GR an important drug target for conditions such as asthma, rheumatoid arthritis, and leukemia.
免疫印迹
WB result of Glucocorticoid Receptor Recombinant Rabbit mAb
Primary antibody: Glucocorticoid Receptor Recombinant Rabbit mAb at 1/500 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: HT-1080 whole cell lysate 20 µg
Lane 5: U-87 MG whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 95 kDaWB result of Glucocorticoid Receptor Recombinant Rabbit mAb
Primary antibody: Glucocorticoid Receptor Recombinant Rabbit mAb at 1/500 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 95 kDaWB result of Glucocorticoid Receptor Recombinant Rabbit mAb
Primary antibody: Glucocorticoid Receptor Recombinant Rabbit mAb at 1/500 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 95 kDaWB result of Glucocorticoid Receptor Recombinant Rabbit mAb
Primary antibody: Glucocorticoid Receptor Recombinant Rabbit mAb at 1/500 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 95 kDa
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