Fibroblast Activation Protein alpha/FAP Recombinant Rabbit mAb (S-3998-2)
Prolyl endopeptidase FAP,170 kDa melanoma membrane-bound gelatinase,Dipeptidyl peptidase FAP,Fibroblast activation protein alpha (FAPalpha),Gelatine degradation protease FAP,Integral membrane serine protease
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宿主来源
Rabbit抗原名称
FAP分子别名
Prolyl endopeptidase FAP; 170 kDa melanoma membrane-bound gelatinase; Dipeptidyl peptidase FAP; Fibroblast activation protein alpha (FAPalpha); Gelatine degradation protease FAP; Integral membrane serine protease免疫原
Recombinant Protein细胞定位
Cell membraneAccession
Q12884克隆号
S-3998-2抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HFF, WI-38纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu IHC-P 1:250 Hu
Fibroblast Activation Protein (FAP) is a 97-kDa type-II transmembrane serine protease that is virtually absent from healthy adult tissues but becomes highly and persistently up-regulated on activated fibroblasts during wound healing, fibrosis, arthritis, atherosclerosis and in the stroma of over 90 % of epithelial cancers, where its unique endopeptidase (Gly-Pro-X) and dipeptidyl-peptidase activities drive extracellular-matrix remodelling, immune evasion, M2 macrophage polarization and tumour-cell proliferation, migration and invasion via signalling axes such as ITGB1/PI3K/AKT and interactions with fibronectin, collagen and immune-checkpoint ligands, making it a powerful prognostic biomarker and an attractive therapeutic target across multiple malignancies and fibrotic diseases.
免疫印迹
WB result of Fibroblast Activation Protein alpha/FAP Recombinant Rabbit mAb
Primary antibody: Fibroblast Activation Protein alpha/FAP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SK-MEL-28 whole cell lysate 20 µg
Lane 2: HFF whole cell lysate 20 µg
Lane 3: WI-38 whole cell lysate 20 µg
Negative control: SK-MEL-28 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 90 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human pancreas. Anti-FAP antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-FAP antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-FAP antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-FAP antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of FAP in tumor tissues.
Expression of FAP in human tissues.
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