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宿主来源
Rabbit抗原名称
Cyclin E2分子别名
G1/S-specific cyclin-E2; CCNE2细胞定位
NucleusAccession
O96020克隆号
S-5247抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
MCF7, HeLa, Jurkat纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:100 Hu ICC 1:500 Hu
Cyclin E2, encoded by the CCNE2 gene, is a cell cycle protein that serves as an activating subunit of CDK2 and CDK3, playing a central regulatory role in the G1-to-S phase transition. Its expression levels and kinase activity peak at the G1/S boundary, driving the initiation of DNA replication through phosphorylation of substrates such as the Rb protein. Compared to its family member Cyclin E1, Cyclin E2 exhibits a unique high-expression pattern in normal tissues such as the testis, thymus, and brain, and is significantly upregulated in various tumor cells, where it has been closely linked to chemotherapy resistance and poor prognosis. As a short-lived protein, its stability is regulated by the ubiquitin-proteasome pathway and precisely controlled by phosphorylation-mediated degradation.
免疫印迹
WB result of Cyclin E2 Recombinant Rabbit mAb
Primary antibody: Cyclin E2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Cyclin E2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-Cyclin E2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Cyclin E2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Cyclin E2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Cyclin E2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- Cyclin E2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Cyclin E2 in tumor tissues.
Expression of Cyclin E2 in human tissues.
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