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Rabbit抗原名称
Thrombospondin 1分子别名
Thrombospondin-1; TSP; TSP1; THBS1免疫原
Synthetic Peptide细胞定位
Endoplasmic reticulum, SecretedAccession
P07996克隆号
S-4153-22抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
mouse platelet, rat platelet纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt IHC-P 1:2000 Hu, Ms, Rt
Thrombospondin-1 (TSP-1) is a multifunctional secreted glycoprotein composed of trimers and belongs to the matricellular protein family within the extracellular matrix. It contains multiple domains that interact with various cell surface receptors (such as CD36, CD47, integrins) and matrix molecules (such as heparin, fibronectin, collagen). Physiologically, TSP-1 is best known as an endogenous angiogenesis inhibitor—it binds to the CD36 receptor, activating p38 MAPK and caspase signaling pathways to directly induce endothelial cell apoptosis, thereby potently suppressing angiogenesis. Additionally, TSP-1 is a key activator of transforming growth factor-beta (TGF-β), releasing active TGF-β through conformational changes to regulate immune responses, fibrosis, and tissue remodeling. Pathologically, dysregulation of TSP-1 expression is associated with various diseases: in the tumor microenvironment, its high expression can inhibit tumor angiogenesis and immune surveillance, though it may also be associated with tumor progression and metastasis. In cardiovascular disease, TSP-1 inhibits nitric oxide signaling via the CD47 pathway, promoting platelet aggregation and thrombosis. In diabetic nephropathy, TSP-1-mediated TGF-β activation drives renal fibrosis. Therefore, TSP-1 serves as a crucial regulatory hub in the extracellular matrix, playing complex and important roles in vascular biology, immune regulation, and fibrotic diseases.
免疫印迹
WB result of Thrombospondin 1 Recombinant Rabbit mAb
Primary antibody: Thrombospondin 1 Recombinant Rabbit mAb at 1/××× dilution
Lane 1: mouse platelet lysate 5 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 129 kDa
Observed MW: 140, 180 kDaWB result of Thrombospondin 1 Recombinant Rabbit mAb
Primary antibody: Thrombospondin 1 Recombinant Rabbit mAb at 1/××× dilution
Lane 1: rat platelet lysate 5 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 129 kDa
Observed MW: 140, 180 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human bone marrow. Anti- Thrombospondin 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti- Thrombospondin 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse bone marrow. Anti- Thrombospondin 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti- Thrombospondin 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat bone marrow. Anti- Thrombospondin 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti- Thrombospondin 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Thrombospondin 1 in tumor tissues.
Expression of Thrombospondin 1 in human tissues.
Expression of Thrombospondin 1 in mouse & rat tissues.
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