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Rabbit抗原名称
NPM1分子别名
Nucleophosmin; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; NPM免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cytoskeleton, NucleusAccession
P06748克隆号
S-4119-58抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
293T, HeLa, Jurkat, HCT 116, MEF, NIH/3T3, 3T3-L1, C2C12, mouse testis, PC-12, rat testis, COS-7预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt, Mk IHC-P 1:2000 Hu, Ms, Rt
Nucleophosmin (NPM1), also known as B23 or numatrin, is a highly abundant, multifunctional phosphoprotein primarily localized in the granular component of the nucleolus, where it plays pivotal roles in ribosome biogenesis, centrosome duplication, and the regulation of genomic stability. As a molecular chaperone with histone-binding activity, NPM1 facilitates the assembly and transport of ribosomal subunits from the nucleolus to the cytoplasm, thereby ensuring efficient protein synthesis, while also participating in the stress response by shuttling between the nucleus and cytoplasm to regulate the stability and activity of key tumor suppressors such as p53 and ARF. Structurally characterized by its ability to form pentameric rings and undergo liquid-liquid phase separation, NPM1 is crucial for maintaining nucleolar architecture; however, its dysfunction, particularly through cytoplasmic mislocalization caused by specific mutations like NPM1c+, is a defining driver event in acute myeloid leukemia (AML), highlighting its dual significance as both an essential housekeeping protein and a critical oncoprotein when deregulated.
免疫印迹
WB result of NPM1 Recombinant Rabbit mAb
Primary antibody: NPM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: HCT 116 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 38 kDaWB result of NPM1 Recombinant Rabbit mAb
Primary antibody: NPM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MEF whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: 3T3-L1 whole cell lysate 20 µg
Lane 4: C2C12 whole cell lysate 20 µg
Lane 5: mouse testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 38 kDaWB result of NPM1 Recombinant Rabbit mAb
Primary antibody: NPM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 38 kDaWB result of NPM1 Recombinant Rabbit mAb
Primary antibody: NPM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 38 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-NPM1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-NPM1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-NPM1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-NPM1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-NPM1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-NPM1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of NPM1 in tumor tissues.
Expression of NPM1 in human tissues.
Expression of NPM1 in mouse & rat tissues.
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