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NLRP3 Recombinant Rabbit mAb (S-3914-9)

NACHT,LRR and PYD domains-containing protein 3,Cold autoinflammatory syndrome 1 protein homolog,Cryopyrin,Mast cell maturation-associated-inducible protein 1,PYRIN-containing APAF1-like protein 1,Cias1,Mmig1,Nalp3,Pypaf1,Nlrp3

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B60061
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    NLRP3

  • 分子别名

    NACHT, LRR and PYD domains-containing protein 3; Cold autoinflammatory syndrome 1 protein homolog; Cryopyrin; Mast cell maturation-associated-inducible protein 1; PYRIN-containing APAF1-like protein 1; Cias1; Mmig1; Nalp3; Pypaf1; Nlrp3

  • 细胞定位

    Cytoskeleton, Nucleus, Mitochondrion, Secreted, Endoplasmic reticulum, Cytoplasm

  • Accession

    Q8R4B8

  • 克隆号

    S-3914-9

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Ms

  • 阳性样本

    RAW264.7 treated with 10 µg/ml LPS for 8 hours

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Ms
背景介绍

  • NLRP3 (NOD-, LRR- and pyrin domain-containing protein 3) is a ~118 kDa cytosolic pattern-recognition receptor that assembles into the macromolecular “NLRP3 inflammasome” when confronted by a structurally diverse set of microbial toxins, RNA viruses, extracellular ATP, crystalline substances or metabolic danger signals; upon activation NLRP3 nucleates the adaptor ASC and pro-caspase-1 filaments, driving caspase-1–mediated proteolytic maturation of interleukin-1β/18 and gasdermin D, which triggers pyroptotic cell death and amplifies systemic inflammation, thereby placing NLRP3 at the hub of innate immunity, autoinflammatory syndromes and chronic metabolic disorders such as gout, atherosclerosis, Alzheimer’s disease and type-2 diabetes.

  • 免疫印迹

    • WB result of NLRP3 Recombinant Rabbit mAb
      Primary antibody: NLRP3 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated RAW264.7 whole cell lysate 20 µg
      Lane 2: RAW264.7 treated with 10 µg/ml LPS for 8 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 118 kDa
      Observed MW: 118 kDa

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